南京医科大学学报（自然科学版）2026，Vol.46Issue(8)：1175-1186,12.DOI:10.7655/NYDXBNSN260281
基于OPA三元缩合反应的多功能抗菌水凝胶敷料的构建及体外性能研究
Construction and in vitro evaluation of a multifunctional antibacterial hydrogel dressing based on OPA-mediated ternary condensation
摘要
Abstract
Objective:This study aimed to develop an antibiotic-free,multifunctional antibacterial hydrogel dressing that integrates rapid gelation,robust tissue adhesion,controllable self-degradation,fluorescence-enabled visualization,and broad-spectrum antibacterial activity,and to systematically evaluate its in vitro performance.Methods:An ultrafast in situ-forming isoindole-crosslinked hydrogel dressing,designated OSN,was fabricated through a ternary condensation reaction initiated by mixing precursor solutions of o-phthalaldehyde(OPA),four-arm poly(ethylene glycol)thiol(tetra-PEG-SH),and four-arm poly(ethylene glycol)amine(tetra-PEG-NH2).By varying the molar ratio of OPA to the two polymer precursors(0.5∶1∶1,1∶1∶1,and 2∶1∶1),three hydrogels,denoted OSN-1,OSN-2,and OSN-3,were prepared.Their gelation behavior,microstructure,mechanical properties,self-degradation profile,biocompatibility,and antibacterial activity were systematically characterized using scanning electron microscopy,rheological analysis,mechanical testing,UV-visible/fluorescence spectroscopy,cell-based assays(including CCK-8,live/dead staining,and scratch wound assays)and antibacterial evaluations(including colony counting,scanning electron microscopy,biofilm staining and flow cytometry).Results:The OSN hydrogels formed rapidly in situ within 10 s and exhibited a homogeneous three-dimensional network architecture.Increasing the OPA content accelerated gelation and imparted self-degradable behavior to the hydrogel matrix,with OSN-3 undergoing complete degradation within 24 h.The hydrogels demonstrated favorable tissue-adhesive strength,together with good biocompatibility.They also promoted the migration of L929 fibroblasts and exhibited potent antibacterial activity against both methicillin-resistant Staphylococcus aureus and Escherichia coli,effectively disrupting bacterial biofilms.In addition,degradation of the hydrogel was accompanied by attenuation and eventual disappearance of blue fluorescence,attributable to cleavage of the isoindole ring,indicating the potential for fluorescence-based visualization of the degradation process.Conclusion:This study successfully developed an antibiotic-free antibacterial OSN hydrogel dressing based on OPA-mediated ternary condensation.The resulting dressing combines injectability,rapid gelation,strong tissue adhesion,controllable self-degradation,fluorescence-enabled visualization,and broad-spectrum antibacterial activity,while maintaining good biocompatibility.These findings provide a novel and promising strategy for the development of antibiotic-free intelligent wound-management strategies.关键词
OPA三元缩合反应/自降解/抗菌水凝胶敷料/异吲哚结构/感染性伤口Key words
OPA-mediated ternary condensation/self-degradation/antibacterial hydrogel dressing/isoindole structure/infected wounds分类
医药卫生引用本文复制引用
沈世彬,晏美俊,王星,唐国柯..基于OPA三元缩合反应的多功能抗菌水凝胶敷料的构建及体外性能研究[J].南京医科大学学报（自然科学版）,2026,46(8):1175-1186,12.基金项目
甘肃省科技计划项目(自然科学基金)(24JRRF008) （自然科学基金）
青岛市医疗卫生科研项目(2024-WJKY193) （2024-WJKY193）
酒泉市科技计划项目(2025MA1020) （2025MA1020）
