摘要
Abstract
[Objective]To obtain an extraction process with high content of effective components,suitable for large-scale industrial production,simple operation,and controllable cost,so as to provide technical support for subsequent formulation development and pharmacological research of Eugenia caryophyllata Thunb.and Syzygium aromaticum(L.)Merr.&L.M.Perry extracts.[Method]Taking the content of eugenol and bancroftinone in the extract(i.e.,the extract phenol rate)as the core evaluation index,the process efficiency of four extraction methods:water vapor distillation,ultrasonic extraction,Soxhlet extraction,and ultrasonic-assisted distillation with enzyme assistance,was compared using high-performance liquid chromatography(HPLC).The best extraction method was selected.Based on this,by single-factor experiments of particle size,liquid-to-solid ratio,and soaking time combined with L9(34)orthogonal experimental design,the water vapor distillation process was optimized,and the optimal extraction process was verified.[Result]By comparison of the four extraction methods,the extract obtained by water vapor distillation had a significantly higher phenol content than those of the other traditional methods.The results of orthogonal experimental variance analysis showed that there was no significant difference in the phenol content among the three factors(particle size,liquid-to-solid ratio,and soaking time)within the set gradient of the experiment(P＞0.05,α=0.05);based on the results of orthogonal experiments and comprehensive consideration of industrial production energy consumption,the optimal process conditions for water vapor distillation of eugenol were determined as:particle size 80 mesh,liquid-to-solid ratio 1∶14(g/mL),and soaking time 6 h;the optimal process conditions for bancroftinone were:particle size 80 mesh,liquid-to-solid ratio 1∶12(g/mL),and soaking time 24 h.[Conclusion]The verification test confirmed that under the above optimized process conditions,the amount of target effective components in Eugenia caryophyllata Thunb.and Syzygium aromaticum(L.)Merr.&L.M.Perry extracts was the highest.This process is stable and has good repeatability,and can be used as a standardized industrial process for efficient extraction of eugenol and bancroftinone.关键词
丁香酚/母丁香酚/提取工艺/正交试验法Key words
Eugenol/Bancroftinone/Extraction process/Orthogonal experimental method分类
医药卫生
