四川林业科技2026，Vol.47Issue(4)：21-30,10.DOI:10.12172/202605110002
热带顽拗性植物遗传转化与基因编辑技术研究进展
Research progress on genetic transformation and gene editing technologies for tropical recalcitrant plants
摘要
Abstract
Tropical recalcitrant plants refer to tropical plants that exhibit significant resistance to in vitro culture and genetic transformation,with their breeding improvement being limited by low regeneration efficiency and strong genotype dependence.In recent years,the coordinated development of CRISPR/Cas-based genome editing and diversified genetic transformation systems has opened up a new way to break through this bottleneck.In this article,the physiological characteristics and biological basis of regeneration obstacles in tropical recalcitrant plants were systematically reviewed,and the internal mechanisms such as the restricted expression of cell totipotency,the activation of defense responses,and the imbalance of molecular regulatory networks,were deeply analyzed.The latest progress of two key transformation strategies,tissue culture-dependent and in situ transformation systems without tissue culture,was reviewed emphatically.Meanwhile,the evolution trend of gene editing delivery systems was summarized.Finally,the development prospects of molecular breeding of tropical recalcitrant plants was propected,and it was proposed that future research should be based on the species-specific biological laws,adopt targeted technical strategies,and pay attention to the collaborative application of various technical routes.关键词
热带顽拗性植物/再生能力/分子育种/遗传转化/基因编辑Key words
tropical recalcitrant plant/regeneration ability/molecular breeding/genetic transformation/gene editing引用本文复制引用
孙佳乐,王立凤,郑艳..热带顽拗性植物遗传转化与基因编辑技术研究进展[J].四川林业科技,2026,47(4):21-30,10.基金项目
云南重大科技规划项目(202402AE090019) （202402AE090019）
中国科学院战略优先研究计划(XDB1080000) （XDB1080000）
西双版纳科技局技术创新专项项目(2025kjcx003) （2025kjcx003）
国际自然科学基金(32200306). （32200306）
