山东科学2026，Vol.39Issue(4)：27-35,96,10.DOI:10.3976/j.issn.1002-4026.2025068
地龙提取物减轻炎症缓解斑马鱼血栓形成
Pheretima extract relieves inflammation and thrombosis in zebrafish
摘要
Abstract
This study investigated the anti-thrombotic activity and underlying mechanisms of action of Pheretima extract(Phe)based on a zebrafish model.A Ponatinib(PON)-induced zebrafish thrombosis model was used to evaluate the anti-thrombotic activity of Phe by measuring heart rate and blood flow velocity.The transgenic zebrafish line Tg(CD41:eGFP),with fluorescently labeled platelets,was used to quantify circulating platelets in vivo,while the macrophage-labeled transgenic line Tg(coro1a:EGFP)was used to assess the effects of Phe on inflammatory responses.In addition,quantitative real-time PCR(qRT-PCR)was used to examine the impact of the Phe on the expression of related target genes.The results showed that Phe significantly improved multiple pathological indicators in the PON-induced zebrafish thrombosis model:it effectively restored drug-induced reductions in heart rate,improved tail blood flow velocity,alleviated inflammatory responses,and reduced the consumption of circulating platelets.Further quantitative PCR(qPCR)analysis indicated that Phe,as the primary active component of Qilong capsules,effectively inhibited the upregulation of multiple coagulation-(VWF,f2,fga,fgb,and tbxas1)and inflammation-related genes(NF-κB and IL-1β)induced by PON.These findings suggest that Phe might exert anti-thrombotic effects by regulating the coagulation cascade,inhibiting platelet activation and aggregation,and suppressing inflammatory response.关键词
斑马鱼模型/血栓形成/地龙提取物/炎症缓解Key words
zebrafish model/thrombosis/Pheretima extract/inflammation relief分类
医药卫生引用本文复制引用
陈佳瑜,高松英,展文,杜兴硕,李晓彬,夏青,何秋霞..地龙提取物减轻炎症缓解斑马鱼血栓形成[J].山东科学,2026,39(4):27-35,96,10.基金项目
山东省重点研发计划(2022CXGC020515) （2022CXGC020515）
