药学研究2026，Vol.45Issue(7)：762-768,7.DOI:10.13506/j.cnki.jpr.2026.07.007
抗肿瘤活性化合物胡桃醌脂质体的构建与评价
Construction and evaluation of juglone liposomes as an antitumor active compound
吴倩 1戴玉梅 2段美涛 2任君刚 2史伟国1
作者信息
- 1. 佳木斯大学药学院,黑龙江 佳木斯 154007
- 2. 厦门医学院机能与临床转化福建省高等学校重点实验室,福建 厦门 361023
- 折叠
摘要
Abstract
Objective To prepare juglone liposomes to enhance the stability and water solubility of juglone.Methods Juglone liposomes were prepared using the thin-film dispersion method.A relatively optimal formulation was preliminarily screened through single-factor experiments,followed by an orthogonal test design.With the entrapment efficiency(EE)as the evaluation index,the effects of membrane material ratio(soybean phospholipid:cholesterol),lipid-to-drug ratio(membrane materials:juglone),organic solvent volume,and ultrasonic temperature on the entrapment efficiency were investigated to determine the optimal preparation process for juglone liposomes.A high-performance liquid chromatography(HPLC)method was established for the determination of juglone content,and its entrapment efficiency and drug loading(DL)were calculated.Quality evaluation was performed on the optimized juglone liposomes.Results The optimal formulation of juglone liposomes was lipid to cholesterol ratio of 5∶1,lipid material to juglone ratio of 10∶1,40 mL organic solvent volume,and ultrasound temperature of 4℃.The prepared juglone liposomes were brownish-yellow liquid with good fluidity,an average particle size of 327.6 nm,a polydispersity index(PDI)of 0.016,and an average Zeta potential of-48.4 mV.The average encapsulation efficiency of juglone was 96.91%,with an RSD of 0.05%,and the average drug loading was 6.317%,with an RSD of 0.02%.The leakage rate of juglone liposomes after being placed at 4℃for 30 days was 4.72%.Conclusion This study established a stable preparation process for juglone liposomes,enhancing both the stability and solubility of juglone.关键词
胡桃醌/脂质体/薄膜分散法/正交试验/多分散性系数/包封率/载药量Key words
Juglone/Liposomes/Thin film dispersion method/Orthogonal design/PDI/Encapsulation efficiency/Drug loading分类
医药卫生引用本文复制引用
吴倩,戴玉梅,段美涛,任君刚,史伟国..抗肿瘤活性化合物胡桃醌脂质体的构建与评价[J].药学研究,2026,45(7):762-768,7.
