陕西中医2026，Vol.47Issue(8)：1011-1017,7.DOI:10.3969/j.issn.1000-7369.2026.08.001
电针干预GDNF基因修饰神经干细胞对大鼠脊髓损伤后Caspase-8表达的影响
Effect of electroacupuncture intervention on GDNF gene-modified neural stem cells on expression of Caspase-8 in rats after spinal cord injury
摘要
Abstract
Objective:To explore methods for in vitro isolation and culture of high-purity neural stem cells(NSCs)and their biological characteristics,and to evaluate intervention effects of electroacupuncture combined with glial cell line-derived neurotrophic factor(GDNF)gene-modified NSC transplantation on cell apoptosis and motor function in rats after spinal cord injury.Methods:Fetal mouse brain tissues were isolated for in vitro NSC culture,and lentivirus carrying the GDNF gene was used for infection to assess transfection efficiency and cell growth status.T11 segment spinal cord incomplete transection model was established in 60 adult male SD rats,which were randomly di-vided into normal,model,NSC transplantation,and electroacupuncture intervention groups.BBB score was used to dynamically evaluate hindlimb motor function recovery.Samples were collected at different time points for assess-ment of Caspase-8 expression using immunohistochemistry and western blot.Results:A stable in vitro NSC culture system was successfully established,and primary cells showed good proliferation ability and multipotent differentia-tion potential.Behavioral evaluation showed that both NSC transplantation group and electroacupuncture intervention group had increased BBB scores compared with the model control group,and difference between the two groups was not statistically significant(P＞0.05).Apoptosis marker analysis showed that compared with the model control group,Caspase-8 expression levels were significantly downregulated in both the NSC transplantation group and the electroacupuncture intervention group(P＜0.05),with greater decrease in the electroacupuncture group(P＜0.05).Con-clusion:NSCs cultured in vitro can maintain stable proliferation and differentiation potential within passages 2-3.GDNF gene-modified NSC transplantation can effectively inhibit cell apoptosis after spinal cord injury.Electroacupuncture could further enhance anti-apoptotic effect,although no significant advantage in improving motor function has been observed yet.关键词
脊髓损伤/神经干细胞/电针/胶质细胞源性神经营养因子/细胞凋亡Key words
Spinal cord injury/Neural stem cells/Electroacupuncture/Glial cell line-derived neurotrophic fac-tor/Apoptosis分类
医药卫生引用本文复制引用
雍晨,谭龙旺,陈斐,刘闯,陈仕强,王国毓,李江..电针干预GDNF基因修饰神经干细胞对大鼠脊髓损伤后Caspase-8表达的影响[J].陕西中医,2026,47(8):1011-1017,7.基金项目
国家自然科学基金资助项目(81774349) （81774349）
陕西省科技厅项目(2018SF-348) （2018SF-348）
陕西省中医药管理局项目(2021-ZZ-LC027) （2021-ZZ-LC027）
陕西省重点研发计划项目(2023-YBSF-526) （2023-YBSF-526）
中医药传承创新暨"秦药"开发重点科学研究项目(2021-03-ZZ-008) （2021-03-ZZ-008）
陕西省咸阳市科学技术局重点研发项目(L2023-ZDYF-SF-045) （L2023-ZDYF-SF-045）
