陕西中医2026，Vol.47Issue(8)：1030-1038,9.DOI:10.3969/j.issn.1000-7369.2026.08.004
宁神安脏饮对失眠大鼠下丘脑生物钟基因表达的影响
Effects of Ningshen Anzang Yin on expression of hypothalamic circadian clock genes in insomnia model rats
摘要
Abstract
Objective:To investigate effects of Ningshen Anzang Yin on expression of hypothalamic circadian clock genes in insomnia model rats.Methods:Eighty Sprague-Dawley(SD)rats were randomly divided into four groups blank control group,model group,Ningshen Anzang Yin group,and eszopiclone group,with 20 rats in each group.Except for the blank control group,the other groups were subjected to chronic unpredictable mild stress(CUMS)combined with intraperitoneal injection of para-chlorophenylalanine(PCPA)to establish an insomnia model.The Ningshen Anzang Yin group received 2.36 g/(kg·d)by gavage,the eszopiclone group received 0.32 mg/(kg·d)by gavage,while the blank and model group received an equal volume of 0.9％saline solution daily for 14 consecutive days.General condition and body weight were observed.Sleep latency and duration were assessed using pentobarbital sodium-induced sleep tests.Neuronal morphology in the hypothalamus was examined via hematoxylin-eosin(HE)staining.mRNA expression levels of CLOCK,BMAL1,Per1,and Cry1 in the hypothalamus were detected by qRT-PCR.Protein expression was analyzed by Western blot and immunohistochemistry.Results:Compared with the blank control group,model rats showed poor general condition,reduced body weight(P＜0.05),shortened sleep duration,and vacuolar degeneration of hypothalamic neurons.Compared with the model group,both the Ningshen Anzang Yin and eszopiclone group exhibited improved general condition,increased body weight(P＜0.05),prolonged sleep dura-tion,and ameliorated neuronal morphology.In the model group,mRNA and protein expression of CLOCK and BMAL1 was elevated,whereas that of Per1 and Cry1 was decreased(P＜0.01).Both treatment groups reversed these abnormalities(P＜0.05).The Ningshen Anzang Yin group significantly reversed the protein expression of CLOCK,Per1,and Cry1(P＜0.01),while the eszopiclone group improved CLOCK and Per1 protein expression(P＜0.01).Immunohistochemical analysis revealed enhanced positive expression of CLOCK,BMAL1,Per1,and Cry1 in both treated groups(P＜0.01).Conclusion:Ningshen Anzang Yin can improve behavioral status and sleep quality in in-somnia model rats,possibly through regulation of hypothalamic circadian clock gene expression.关键词
失眠/宁神安脏饮/生物钟基因/昼夜节律/下丘脑/睡眠障碍Key words
Insomnia/Ningshen Anzang Yin/Circadian clock genes/Circadian rhythm/Hypothalamus/Sleep disorders分类
医药卫生引用本文复制引用
袁博,付慧玲,李国花,李东升,贾孟辉,王晓丽..宁神安脏饮对失眠大鼠下丘脑生物钟基因表达的影响[J].陕西中医,2026,47(8):1030-1038,9.基金项目
宁夏回族自治区自然科学基金资助项目(2024AAC03795) （2024AAC03795）
第七批全国老中医药专家学术经验继承项目(国中医教函[2022]76号) （国中医教函[2022]76号）
宁夏回族自治区青年拔尖人才培养项目(宁人社函[2026]33号) （宁人社函[2026]33号）
