上海交通大学学报（医学版）2026，Vol.46Issue(7)：868-874,7.DOI:10.3969/j.issn.1674-8115.2026.07.005
迷迭香酸基水凝胶的促肌腱愈合效应
Promotion of tendon healing by rosmarinic acid-based hydrogel
摘要
Abstract
Objective·To construct a rosmarinic acid(RA)-loaded hydrogel and investigate its effects on tissue repair and functional recovery after Achilles tendon injury.Methods·Phenylboronic acid-modified carboxymethyl chitosan(CMCS-PBA)was synthesized via an amidation reaction between phenylboronic acid and carboxymethyl chitosan,and the molecular structure of CMCS-PBA was confirmed by 1H nuclear magnetic resonance(1H-NMR)spectroscopy.CMCS-PBA/RA hydrogel was prepared by mixing CMCS-PBA with RA at a volume ratio of 10∶1.CMCS-PBA/PVA hydrogel composed of CMCS-PBA and polyvinyl alcohol(PVA)served as the positive control.The rheological property,self-healing ability,and mechanical performance of the hydrogel were systematically characterized with a rheometer.Rat fibroblast cell line 208F was used to evaluate the biocompatibility of the hydrogels via live/dead cell staining.Eighteen rats with established Achilles tendon transection models were randomly assigned into three groups(n=6 per group):the control group,the CMCS-PBA/RA group,and the CMCS-PBA/PVA group.In the CMCS-PBA/RA and CMCS-PBA/PVA groups,the corresponding hydrogels were locally injected into the injured tendons immediately after model establishment to completely cover the injury site,while no hydrogel intervention was administered in the control group.All rats were sacrificed 3 weeks postoperatively.Hematoxylin-eosin(HE)staining and Masson staining were performed to evaluate tendon healing,and gait analysis was conducted to assess functional recovery.Major visceral organs were harvested for HE staining to further verify the in vivo biocompatibility of the prepared hydrogels.Results·1H-NMR results confirmed the successful grafting of PBA onto the CMCS molecular chains.Rheological characterization demonstrated that the prepared hydrogels possessed excellent shear-thinning behavior,self-healing ability,and favorable injectability.Live/dead staining and organ HE staining verified the favorable biocompatibility of the hydrogels.Compared with the control and CMCS-PBA/PVA groups,the CMCS-PBA/RA group exhibited significantly lower tendon healing scores(both P<0.05),indicating superior tendon healing.Gait analysis showed a significantly larger footprint area in the CMCS-PBA/RA group(both P<0.05),which reflected better functional recovery.Conclusion·A novel CMCS-PBA/RA composite hydrogel was successfully prepared.The hydrogel possesses excellent injectability,self-healing capability,and biocompatibility,and effectively promotes Achilles tendon tissue repair and motor functional recovery.关键词
迷迭香酸/水凝胶/肌腱损伤/肌腱修复/可注射/自愈合Key words
rosmarinic acid/hydrogel/tendon injury/tendon repair/injectability/self-healing分类
医药卫生引用本文复制引用
王缘,吴容浦,王非,刘珅..迷迭香酸基水凝胶的促肌腱愈合效应[J].上海交通大学学报（医学版）,2026,46(7):868-874,7.基金项目
国家重点研发计划(2024YFC2418105) （2024YFC2418105）
国家自然科学基金(82572709) （82572709）
上海交通大学医学院"双百人"项目(20191829). National Key Research and Development Program of China(2024YFC2418105) （20191829）
National Natural Science Foundation of China(82572709) （82572709）
"Two-hundred Talents"Program of Shanghai Jiao Tong University School of Medicine(20191829). （20191829）
