世界中医药2026，Vol.21Issue(9)：1645-1652,8.DOI:10.3969/j.issn.1673-7202.2026.09.006
基于转录组学探讨白芍调控细胞因子免疫调节的作用机制
Immunoregulatory Mechanisms of Paeoniae Radix Alba via Cytokine Modulation Based on Transcriptomics
摘要
Abstract
Objective:To investigate the ameliorative effects of Paeoniae Radix Alba on immunosuppressed mice and its potential mechanisms.Methods:Ninety male Kunming(KM)mice were randomly divided into a blank group,model group,ginseng group(0.5 g/kg),and low-,medium-,and high-dose groups of Paeoniae Radix Alba aqueous extract(463.3,926.7,and 1 390.0 mg/kg,respectively)according to a random number table,with continuous administration for 30 days.An immunosuppressed mouse model was established by intraperitoneal injection of cyclophosphamide(80 mg/kg)on days 12 and 13.Body weight,spleen index,thymus index,splenic lymphocyte transformation,NK cell activity,serum immunoglobulin G and M(IgG,IgM),and complement components 3 and 4(C3,C4)were measured.Differentially expressed genes were identified by transcriptome sequencing and sub-jected to enrichment analysis,and key targets were validated by real-time quantitative PCR(RT-qPCR)and Western blot(WB).Results:Compared with the blank group,the model group showed decreased body weight on day 30,reduced thymus and spleen in-dices,decreased lymphocyte transformation,NK cell activity,and reduced levels of IgG,IgM,C3,and C4(P＜0.05).Compared with the model group,these indices were increased in all treatment groups(P＜0.05).Transcriptome sequencing revealed that the cytokine-cytokine receptor interaction pathway was a key signaling pathway.Interleukin-1 receptor-like 1(IL1RL1),tumor necrosis factor ligand superfamily member 12(TNFSF12),chemokine(C-C motif)ligand 24(CCL24),and interleukin-2 receptor subunit beta(IL2RB)were identified as key targets.Compared with the blank group,the model group showed increased IL1RL1 mRNA and protein expression and TNFSF12 mRNA expression,and decreased IL2RB mRNA and protein expression(P＜0.05).Compared with the model group,the high-dose Paeoniae Radix Alba aqueous extract group showed decreased IL1RL1 mRNA and protein ex-pression and TNFSF12 mRNA expression,and increased IL2RB mRNA and protein expression(P＜0.05).Conclusion:Paeoniae Radix Alba exerts immunoprotective effects in immunosuppressed mice.Its immunoregulatory mechanism is associated with modula-tion of IL1RL1,TNFSF12,and IL2RB expression and regulation of the cytokine-cytokine receptor interaction pathway.关键词
白芍/免疫调节/转录组学/细胞因子-细胞因子受体相互作用/白细胞介素-1受体样1/肿瘤坏死因子样弱凋亡诱导物/白细胞介素细胞2受体亚基βKey words
Paeoniae Radix Alba/Immunoregulation/Transcriptomics/Cytokine-cytokine receptor interaction/IL1RL1/TNFSF12/IL2RB分类
医药卫生引用本文复制引用
邸松蕊,谢辰雨,杨霄楠,金相伯,胡培丽,朱映黎..基于转录组学探讨白芍调控细胞因子免疫调节的作用机制[J].世界中医药,2026,21(9):1645-1652,8.基金项目
国家自然科学基金项目(82474097) （82474097）
国家重点研发计划"中医药现代化"研究重点专项(2018YFC1706800). （2018YFC1706800）
