世界中医药2026，Vol.21Issue(9)：1662-1668,7.DOI:10.3969/j.issn.1673-7202.2026.09.008
当归红芪超滤物调控组蛋白去乙酰化酶3对心肌细胞衰老的机制研究
Mechanisms of Angelicae Sinensis Radix and Hedysari Radix Ultrafiltrate in Regulating Histone Deacetylase 3 on Cardiomyocyte Aging
摘要
Abstract
Objective:To investigate the mechanism by which Angelicae Sinensis Radix-Hedysari Radix ultrafiltrate(RAS-RH)regulates histone deacetylase 3(HDAC3)expression to intervene in X-ray-induced senescence of human cardiomyocytes AC16.Methods:AC 16 cells were randomly divided into a normal group,a negative control group(0 Gy X-ray irradiation+0.5 mL of sa-line),a model group(6 Gy X-ray irradiation+0.5 mL of saline),a low-dose RAS-RH group(6 Gy X-ray irradiation+0.5 mL of low-dose drug-containing serum),a medium-dose RAS-RH group(6 Gy X-ray irradiation+0.5 mL of medium-dose drug-containing serum),and a high-dose RAS-RH group(6 Gy X-ray irradiation+0.5 mL of high-dose drug-containing serum).After intervention,cell proliferation,antioxidant capacity,and cellular senescence were evaluated in each group.The expression levels of transforming growth factor-β1(TGF-β1),Smad3,histone deacetylase 3(HDAC3),and p21 were detected.Results:Compared with the negative control group,AC16 cell viability in the model group was significantly decreased(P＜0.01).The protein expression levels of TGF-β1,Smad3,HDAC3,and p21 were significantly upregulated in the model group(P＜0.05).The proportion of senescence-positive cells was significantly increased(P＜0.01),while total antioxidant capacity(TAC)was significantly reduced(P＜0.05).Com-pared with the model group,the expression levels of TGF-β1,Smad3,HDAC3,and p21 were significantly downregulated in the low-,medium-,and high-dose RAS-RH groups(P＜0.05),with the high-dose group showing the most pronounced reduction.Senes-cence-positive staining was markedly decreased in the high-dose group(P＜0.01).TAC was significantly increased in the medium-and high-dose groups(P＜0.05).Conclusion:RAS-RH can attenuate X-ray-induced senescence in AC 16 cells by inhibiting acti-vation of the TGF-β1/Smad3 signaling pathway and downregulating HDAC3 expression.关键词
当归红芪超滤物/放射性心肌损伤/心肌纤维化/细胞衰老/转化生长因子-β1/Sma和Mad相关蛋白同源物3/组蛋白去乙酰化酶3Key words
Angelicae Sinensis Radix-Hedysari Radix ultrafiltrate/Radioactive myocardial injury/Myocardial fibrosis/Cell se-nescence/TGF-β1/Smad3/HDAC3分类
医药卫生引用本文复制引用
陈玉林,赵信科,侯红叶,蒋虎刚,王新强,赵晓彬,韩金晏,刘佳坤,安涛,李应东..当归红芪超滤物调控组蛋白去乙酰化酶3对心肌细胞衰老的机制研究[J].世界中医药,2026,21(9):1662-1668,7.基金项目
国家自然科学基金项目(82360926) （82360926）
甘肃省卫生健康行业科研项目(GSWSQN2025-10) （GSWSQN2025-10）
甘肃省中医药科研课题(GZKP-2023-59) （GZKP-2023-59）
甘肃省中医药研究中心/甘肃省中医方药挖掘与创新转化重点实验室开放基金项目(FYWJ-24-04). （FYWJ-24-04）
