世界中医药2026，Vol.21Issue(9)：1669-1676,8.DOI:10.3969/j.issn.1673-7202.2026.09.009
基于肠道菌群探讨小柴胡汤加味方对胃癌前病变大鼠的作用及机制
Effects and Mechanisms of Modified Xiaochaihu Decoction on Precancerous Lesion of Gastric Cancer in Rats Based on Gut Microbiota
摘要
Abstract
Objective:To investigate the interventional effects of modified Xiaochaihu Decoction on rats with precancerous lesion of gastric cancer(PLGC),and to explore its mechanisms based on gut microbiota.Methods:Sixty rats were randomly divided into six groups using a random number table method.Except for the blank group,PLGC was induced in all other groups by free access to 200 mg/L 1-methyl-3-nitro-1-nitrosoguanidine(MNNG)solution combined with an irregular feeding regimen(alternating fasting and overeating)for 18 weeks.After successful modeling,rats in the blank and model groups were administered saline by gavage,the positive control group received a suspension of folic acid tablets and rebamipide tablets,and the low-,medium-,and high-dose mod-ified Xiaochaihu Decoction groups received corresponding concentrations of the herbal formula by gavage for 12 weeks.Histopatho-logical changes in gastric mucosa were observed using hematoxylin-eosin(HE)staining.Serum levels of pepsinogen Ⅰ(PG Ⅰ),pepsinogen Ⅱ(PG Ⅱ),gastrin-17(GAS-17),MG7-Ag,and carcinoembryonic antigen(CEA)were measured by enzyme-linked im-munosorbent assay(ELISA).Gut microbiota changes were analyzed using 16S ribosomal DNA(16S rDNA)sequencing.Correlation analysis was performed using Pearson correlation analysis.Results:Compared with the blank group,rats in the model group showed abnormal gastric tissue structure,obvious mucosal lesions,partial atrophy with epithelial atypia,and inflammatory cell infiltration.These pathological changes were alleviated in the modified Xiaochaihu Decoction groups.Serum levels of PG Ⅰ,PG Ⅱ,GAS-17,MG7-Ag,and CEA were significantly improved.In addition,gut microbiota diversity and composition were optimized.Dominant bac-terial genera were correlated with serum levels of gastric function indicators and tumor-related factors in PLGC rats.Conclusion:Modified Xiaochaihu Decoction may improve gastric mucosal pathological changes by regulating gut microbiota and influencing the expression of gastric function-related and tumor-related factors in PLGC rats.关键词
小柴胡汤加味方/胃癌前病变/肠道菌群/16S核糖体DNA/胃功能/肿瘤相关因子/优势菌属Key words
Modified Xiaochaihu Decoction/PLGC/Gut microbiota/16S rDNA/Gastric function/Tumor-related factors/Domi-nant bacterial genera分类
医药卫生引用本文复制引用
景燕燕,梅汉玮,宋云升,白湘玉,杨志鹏,袁红霞,刘军舰,袁旭..基于肠道菌群探讨小柴胡汤加味方对胃癌前病变大鼠的作用及机制[J].世界中医药,2026,21(9):1669-1676,8.基金项目
国家自然科学基金项目(82505555,82204962) （82505555,82204962）
天津市教委科研计划项目(2022KJ271) （2022KJ271）
天津市多元投入基金项目重点项目(21JCZDJC01150) （21JCZDJC01150）
天津市卫生健康委中医中西医结合科研课题(2025111) （2025111）
天津市医学重点学科建设资助项目(TJYXZDXK-3-028C). （TJYXZDXK-3-028C）
