世界中医药2026，Vol.21Issue(9)：1677-1688,12.DOI:10.3969/j.issn.1673-7202.2026.09.010
苍菊清肝降脂方对代谢相关脂肪性肝病小鼠胆汁酸代谢的影响及机制
Effects and Mechanisms of Cangju Qinggan Jiangzhi Formula on Bile Acid Metabolism in Mice with Metabolic Dysfunction-associated Steatotic Liver Disease
摘要
Abstract
Objective:To investigate the effects of Cangju Qinggan Jiangzhi Formula on hepatic injury and bile acid metabolism in mice with metabolic dysfunction-associated steatotic liver disease(MASLD).Methods:Forty C57BL/6J mice were randomly divid-ed into a control group,a model group,an obeticholic acid group,and low-and high-dose Cangju Qinggan Jiangzhi Formula groups(2.3 and 4.6 g/kg)according to a random number table.A MASLD model was established by feeding a high-fat diet for 16 weeks.Body weight,liver wet weight,liver function indices,serum lipid levels,and hepatic triglyceride content were measured.His-topathological changes in liver tissue were evaluated using hematoxylin-eosin(HE)staining and Oil Red O staining.Bile acid pro-files in liver,serum,and feces were determined by ultra-high-performance liquid chromatography-tandem mass spectrometry(UH-PLC-MS/MS).The mRNA and protein expression levels of farnesoid X receptor(FXR),small heterodimer partner(SHP),choles-terol 7α-hydroxylase(CYP7 A1),sterol 27-hydroxylase(CYP27 A1),bile salt export pump(BSEP),and sodium taurocholate co-transporting polypeptide(NTCP)were detected by quantitative real-time PCR(qRT-PCR)and Western blot,respectively.Results:Compared with the control group,the model group showed significant increases in body weight,liver wet weight,liver function indi-ces,and serum lipid levels(P＜0.05),accompanied by accumulation of multiple bile acids in the liver and serum and decreased bile acid excretion in feces(P＜0.05).The mRNA and protein expression levels of FXR,SHP,and BSEP were downregulated(P＜0.05),whereas CYP7 A1 and NTCP were upregulated(P＜0.05).Compared with the model group,intervention with different doses of Cangju Qinggan Jiangzhi Formula significantly improved the above indices in mice(P＜0.05).Conclusion:Cangju Qinggan Jiangzhi Formula alleviates hepatic injury and improves bile acid metabolic imbalance in MASLD mice,and its mechanism may be associated with regulation of FXR/SHP-mediated pathways involved in bile acid synthesis and transport.关键词
苍菊清肝降脂方/代谢相关脂肪性肝病/胆汁酸代谢/法尼醇X受体信号通路/小异二聚体伴侣蛋白/胆汁酸转运蛋白/肝脏脂质沉积/中医药干预分类
医药卫生引用本文复制引用
刘有芳,金源源,石杰文,陈建杰,成扬..苍菊清肝降脂方对代谢相关脂肪性肝病小鼠胆汁酸代谢的影响及机制[J].世界中医药,2026,21(9):1677-1688,12.基金项目
国家自然科学基金面上项目(81774098). （81774098）
