山西医科大学学报2026，Vol.57Issue(7)：794-802,9.DOI:10.13753/j.issn.1007-6611.2026.07.008
淮河沉积物来源黄柄曲霉BMC-28次级代谢产物分离鉴定及其抗氧化活性
Isolation,identification and antioxidant activity of secondary metabolites from Aspergillus flavipes BMC-28 derived from Huaihe River sediment
摘要
Abstract
Objective To isolate and identify the secondary metabolites of the fungus Aspergillus flavipes BMC-28 derived from Huaihe River sediment,and evaluate their antioxidant activity.Methods The monomeric compounds were separated and purified by multiple chromatographic techniques such as normal and reversed-phase silica gel column chromatography.The structural identifica-tion of the compounds was performed by spectroscopic techniques such as nuclear magnetic resonance(NMR)and mass spectrometry(MS).The antioxidant activities of the identified compounds were evaluated using the 1,1-Diphenyl-2-picrylhydrazyl(DPPH)method.Results Fifteen known compounds were isolated and identified from the rice fermentation culture of Aspergillus flavipes BMC-28,including sulochrin(1),2-(3'-hydroxy-2'-(methoxycarbonyl)-5'-methylphenoxy)-4-hydroxy-6-methoxybenzoic acid(2),methyl chloroasterrate(3),asterric acid(4),monomethylosoic acid(5),iizukine B(6),epicoccone(7),epicoccolide B(8),N-ben-zoylphenylalaninyl-N-benzoylphenylalaninate(9),questin(10),(2E,2'E)-[6,6'-dihydroxy[1,1'-biphenyl]-3,3'-diyl]bis[prop-2-enoic acid](11),auranticin A(12),2,6-Dihydroxy-3,4-dimethylbenzoic acid(13),4-Methylorcinol(14),4,5,6-Trimethylbenzene-1,3-diol(15).Among them,compounds 11 and 12 were isolated from the genus Aspergillus for the first time.The in vitro antioxidant experiment results revealed that compounds 6,7 and 8 exhibited strong DPPH radical scavenging activity with half-maximal inhibitory concentration(IC50)of 1.53-3.68 μmol/L,significantly superior to the positive control vitamin C.Structure-activity relationship analysis results indicated that the presence of multiple phenolic hydroxyl substitutions in the molecular structure was an important structural feature for compound to exhibit strong antioxidant activity.Conclusion Fifteen known compounds are successfully isolated and identified from Aspergillus flavipes BMC-28 derived from Huaihe River sediment,among which compounds 6,7,and 8 possess strong antioxidant activity,significantly superior to vitamin C,and compounds 11,12 are reported for the first time.This study provides a theoretical basis and scientific reference for the further study of natural antioxidant lead compounds and the subsequent development and utiliza-tion of freshwater fungal resources.关键词
淮河/淡水真菌/黄柄曲霉/次级代谢产物/抗氧化活性Key words
Huaihe River/freshwater fungi/Aspergillus flavipes/secondary metabolites/antioxidant activity分类
医药卫生引用本文复制引用
姜婧,陈紫宜,肖君,李岚影,吴润宇,从雅娟,王皓天,朱美林..淮河沉积物来源黄柄曲霉BMC-28次级代谢产物分离鉴定及其抗氧化活性[J].山西医科大学学报,2026,57(7):794-802,9.基金项目
安徽省高校自然研究重点项目(2025AHGXZK30205) （2025AHGXZK30205）
安徽省大学生创新创业计划训练项目(S202510367168) （S202510367168）
