时珍国医国药2026，Vol.37Issue(15)：2808-2814,7.DOI:10.70976/j.1008-0805.SZGYGY-2026-1502
通络蠲痹颗粒调控ROS-NLRP3-Caspase-1轴对骨关节炎软骨细胞焦亡的影响
Effects of Tongluo Juanbi Granule(通络蠲痹颗粒)on pyroptosis of osteoarthritis chondro-cytes via the ROS-NLRP3-Caspase1 axis
摘要
Abstract
Objective To explore the effects of Tongluo Juanbi Granules(通络蠲痹颗粒,TLJB)medicated serum on pyroptosis and the reactive oxygen species(ROS)-NOD-like receptor protein 3(NLRP3)-Caspase-1 signaling axis in rabbit chondrocytes at the cellu-lar level,and to study its mechanism of action in preventing and treating osteoarthritis(OA).Methods TLJB medicated serum and blank serum were extracted.Rabbit chondrocytes were cultured in vitro,and divided into normal control group(group A),model group(group B),blank serum group(group C),TLJB medicated serum group(group D),NLRP3 activator group(group E),and TLJB medi-cated serum+NLRP3 activator group(group F).Chondrocytes were identified using Toluidine Blue staining and Type II collagen immu-nofluorescence.MTT assay was used to select the optimal intervention concentration of TLJB medicated serum.Lactate dehydrogenase(LDH)levels were detected by a coupled enzyme assay.Levels of IL-1β and IL-18 were detected by enzyme-linked immunosorbent assay(ELISA).ROS levels were detected by a chemiluminescence method.Real-time quantitative PCR(RT-qPCR)was used to detect the mRNA expression of NLRP3,Caspase-1,GSDMD,IL-1β and IL-18 in chondrocytes.Western blot(WB)was used to detect the protein expression of NLRP3,pro-Caspase-1 and GSDMD.Results MTT assay showed that 15%TLJB medicated serum intervention for 48 hours was optimal for rabbit chondrocyte proliferation.Compared with group A,LDH,IL-1β,IL-18,ROS levels,and mRNA and protein expression of NLRP3,Caspase-1,and GSDMD in group B were significantly increased(P<0.01).Compared with group B,group E showed significant increases in LDH,IL-1β,IL-18,ROS levels,and mRNA and protein expression of NLRP3,Caspase-1,and GSDMD(P<0.01),while group D showed significant decreases in these indicators(P<0.01).Compared with group E,LDH,IL-1β,IL-18,ROS levels,and mRNA and protein expression of NLRP3,Caspase-1,and GSDMD in group F were significantly decreased(P<0.01).Conclusion TLJB medicated serum can inhibit pyroptosis in rabbit articular chondrocytes,and its mechanism may be related to the suppression of the ROS-NLRP3-Caspase1 signaling axis activation.关键词
通络蠲痹颗粒/骨关节炎/软骨细胞/细胞焦亡/ROS-NLRP3-Caspase1Key words
Tongluo Juanbi Granules(通络蠲痹颗粒)/Osteoarthritis/Chondrocyte/Pyroptosis/ROS-NLRP3-Caspase1分类
医药卫生引用本文复制引用
胡伟伟,欧梁,黄旺,翟韶阳,齐麒,谭旭仪,黄彦昌,黄维琛..通络蠲痹颗粒调控ROS-NLRP3-Caspase-1轴对骨关节炎软骨细胞焦亡的影响[J].时珍国医国药,2026,37(15):2808-2814,7.基金项目
国家自然科学基金(82405447,82174414) （82405447,82174414）
贵州省科技厅基础研究项目(黔科合基础-ZK[2022]一般479) （黔科合基础-ZK[2022]一般479）
