时珍国医国药2026，Vol.37Issue(15)：2823-2829,7.DOI:10.70976/j.1008-0805.SZGYGY-2026-1504
桃红四物汤对大鼠肢体缺血-再灌注损伤中miR-675、CaMKII、TNF-α的表达影响及干预作用
Effects of Taohong Siwu Decoction(桃红四物汤)on miR-675,CaMKII,and TNF-α expres-sion in rats with limb ischemia-reperfusion injury
摘要
Abstract
Objective To investigate the intervention effect of Taohong Siwu Decoction(桃红四物汤,THSWD)on the expressions of miR-675,CaMKII,and TNF-α in a rat model of limb ischemia-reperfusion injury(LIRI).Methods Seventy-eight male SD rats were randomly assigned to four groups:the control,model,miR-675 inhibitor,and THSWD groups.Except for the control group,LIRI was induced in the rats of the other groups.After successful modeling,skeletal muscle and serum samples were collected at 0,2,4,and 8 hours for analysis.HE staining was used to observe morphological and pathological changes in skeletal muscle,and DAPI staining was used to assess cell apoptosis.The expression levels of miR-675 and CaMKII mRNA were detected by RT-qPCR.CaMKII protein expres-sion was measured by Western blot(WB),and serum TNF-α level was measured by ELISA.Results HE staining revealed the most severe skeletal muscle injury in the model group,while the injury was alleviated in the miR-675 inhibitor and THSWD groups.DAPI staining indicated that the model group had the highest number of apoptotic cells and the highest apoptosis rate,which was significantly different from the other groups(P<0.05).At each corresponding time point,RT-qPCR and WB analyses showed that the expressions of miR-675 and CaMKII mRNA,as well as CaMKII protein,were significantly higher in the model,miR-675 inhibitor,and THSWD groups than in the control group(P<0.05).However,the expressions in the miR-675 inhibitor and THSWD groups were significantly lower than those in the model group(P<0.05).ELISA revealed that serum TNF-α levels in all experimental groups were higher than those in the control group(P<0.05).Notably,the levels in the miR-675 inhibitor and THSWD groups were significantly lower than those in the model group(P<0.05).Conclusion THSWD alleviates LIRI in rat skeletal muscle.The mechanism may be associated with its regulation of miR-675 expression,thereby modulating the expression of CaMKII and TNF-α and other inflammatory factors.关键词
肢体缺血-再灌注损伤/miR-675/CaMKII/TNF-α/桃红四物汤Key words
Limb ischemia-reperfusion injury/miR-675/CaMKII/TNF-α/Taohong Siwu Decoction(桃红四物汤)分类
医药卫生引用本文复制引用
陈科祥,朱付平,顾启帆..桃红四物汤对大鼠肢体缺血-再灌注损伤中miR-675、CaMKII、TNF-α的表达影响及干预作用[J].时珍国医国药,2026,37(15):2823-2829,7.基金项目
国家自然科学基金(82274541,81674008) （82274541,81674008）
湖南省自然科学基金(2020JJ4070) （2020JJ4070）
湖南省卫生健康委重点课题(202204072465) （202204072465）
