时珍国医国药2026，Vol.37Issue(15)：2830-2836,7.DOI:10.70976/j.1008-0805.SZGYGY-2026-1505
茱萸丸调控PPARγ/LXRα/ABCA1/CPT1α信号通路改善痤疮模型炎性皮损的作用机制
Zhuyu Pill(茱萸丸)regulates the PPARγ/LXRα/ABCA1/CPT1αsignaling pathway to allevi-ate inflammatory lesions in an acne model
摘要
Abstract
Objective The study aimed to investigate the effects of Zhuyu Pill(朱黄丸,ZYP)on the PPARγ/LXRα/ABCA1/CPT1α signaling pathway in a mouse model of acne,and to explore its mechanism of action in treating acne.Methods A mouse model of acne was established by applying oleic acid on the right auricle and subcutaneous injection of Propionibacterium acnes to induce the auricle combined with acne.The modeling period was 2 weeks.Among the 50 successfully modeled ICR mice,they were randomly divided into 5 groups:the model group,the low,medium,and high doses of ZYP groups,and the isotretinoin group,with 10 mice in each group.Additionally,10 healthy ICR mice of the same age were set as the control group,without modeling.The control group and the model group were given equal volumes of sterile distilled water by gavage,while the low,medium,and high doses of ZYP groups were given 130.54,261.08,and 522.16 mg·kg-1·d-1 by gavage respectively,and the isotretinoin group was given 4.55 mg·kg-1·d-1 by gavage once a day.Each group of mice was gavaged for 4 weeks after the administration.After the intervention,the contents of serum SOD,MDA,GSH-Px and CAT were detected.HE staining,immunofluorescence staining of auricular tissue,real-time PCR and Western blot(WB)were used to detect the related genes and proteins of PPARγ/LXRα/ABCA1/CPT1α.Results The pathological changes of the auricle tissue in the model group mice were aggravated;the content of MDA in serum increased,while the levels of SOD,GSH-Px and CAT decreased(P<0.01);the expression levels of PPARγ,LXRα,ABCA1 and CPT1α protein and mRNA in auricle tissue decreased(P<0.01).Compared with the model group,both ZYP and Isotretinoin could improve the pathological changes and oxidative stress indi-cators;the expression levels of the PPARγ/LXRα/ABCA1/CPT1α pathway in auricle tissue increased(P<0.05,P<0.01).Conclusion ZYP can improve sebum secretion and inflammatory skin lesions in acne model mice,and its mechanism may be related to the activation of the PPARγ/LXRα/ABCA1/CPT1α signaling pathway.关键词
茱萸丸/痤疮/炎性皮损/痤疮小鼠模型/PPARγ/LXRα/ABCA1/CPT1α信号通路Key words
Zhuyu Pill(茱萸丸)/Acne/Inflammatory skin lesions/Acne mouse model/PPARγ/LXRα/ABCA1/CPT1α sig-naling pathway分类
医药卫生引用本文复制引用
宋玮,滕政闻,张钟艺,沈涛,肖敏,张永..茱萸丸调控PPARγ/LXRα/ABCA1/CPT1α信号通路改善痤疮模型炎性皮损的作用机制[J].时珍国医国药,2026,37(15):2830-2836,7.基金项目
四川省科技计划项目(2022YFS0381) （2022YFS0381）
