时珍国医国药2026，Vol.37Issue(15)：2852-2856,5.DOI:10.70976/j.1008-0805.SZGYGY-2026-1508
百合地黄汤制备用百合的水洗工艺对王百合苷A与王百合苷B含量的影响研究
Effect of water washing process on the contents of regaloside A and B in Bulbus Lilii for prepa-ration of Baihe Dihuang Decoction(百合地黄汤)
摘要
Abstract
Objective To evaluate the effects of water washing process parameters(bulb segmentation degree,water addition,and soaking time)on the contents of regaloside A and B in Bulbus Lilii,and to provide a basis for optimizing the processing technology of Bulbus Lilii for Baihe Dihuang Decoction(百合地黄汤,BHDHD).Methods An orthogonal experimental design(factor levels:bulb segmentation degree of 1/4 to 1 whole segment,water addition of 3-7 times,and soaking time of 8-12 h)was employed.High-performance liquid chromatography(HPLC)was used to determine the contents of regaloside A and B in Bulbus Lilii before and after water washing.The chromatographic separation was performed on a ZORBAX SB-Aq column with a mobile phase consisting of acetoni-trile-0.1%phosphoric acid aqueous solution(13:87,v/v)at a detection wavelength of 312 nm.The influence of the water washing pro-cess parameters on the contents of the two components was further evaluated by analysis of variance(ANOVA).Results The content of regaloside A and B in unwashed Bulbus Lilii was(3.075±0.060)mg·g-1 and(1.046±0.010)mg·g-1,respectively.After water washing with different parameters,the content of regaloside A ranged from 2.938 to 3.068 mg·g-1,and that of regaloside B ranged from 1.027 to 1.044 mg·g-1.No significant difference was observed compared with the unwashed group(P>0.05).The results of ANOVA showed that bulb segmentation degree,water addition,and soaking time all had significant effects on the contents of the two components(P<0.05 or P<0.01),with bulb segmentation degree being the most significant factor.Additionally,differences in the response trends of regaloside A and B to the different parameters were observed.Conclusion Within the range of water washing parameters investigated,although each factor had a significant effect on the contents of regaloside A and B,the water washing process itself did not result in sig-nificant losses of these compounds.This suggests that the traditional water washing step for Bulbus Lilii in the preparation of BHDHD is not necessary for retaining the target active ingredients.关键词
百合地黄汤/百合水洗/正交试验/高效液相色谱法/王百合苷A/王百合苷BKey words
Baihe Dihuang Decoction(百合地黄汤)/Lily bulb washing processing/Orthogonal experiment/HPLC/Regaloside A/Regaloside B分类
医药卫生引用本文复制引用
张月月,纪丽婕,何庆文,宋玲玲,弓明珠,王彦嵋,王君明..百合地黄汤制备用百合的水洗工艺对王百合苷A与王百合苷B含量的影响研究[J].时珍国医国药,2026,37(15):2852-2856,5.基金项目
国家自然科学基金青年基金(C类)(82505416) （C类）
中原科技创新领军人才项目(254200510005) （254200510005）
河南省高校科技创新团队支持计划(26IRTSTHN020) （26IRTSTHN020）
河南省自然科学基金青年基金(252300423825) （252300423825）
