时珍国医国药2026，Vol.37Issue(15)：2857-2862,6.DOI:10.70976/j.1008-0805.SZGYGY-2026-1509
基于HPLC多成分定量与化学模式识别的不同产地防风质量差异分析
Analysis of quality variations in Radix Saposhnikoviae from different origins based on HPLC multi-component quantification combined with chemical pattern recognition
摘要
Abstract
Objective To clarify the distribution characteristics of chromone constituents in Radix Saposhnikoviae from different ori-gins and to reveal the influence of geographic factors on its quality.Methods Fifty-four samples(18 batches)were collected from six provinces:Heilongjiang,Inner Mongolia,Hebei,Shaanxi,Gansu,and Henan.The contents of three chromones—prim-O-glucosylcimifugin,cimifugin,and 5-O-methylvisammioside—were determined by HPLC.Multidimensional quality evaluation was per-formed using principal component analysis(PCA),hierarchical cluster analysis(HCA),and grey relational analysis(GRA).Results Significant geographic variations were observed in the three chromone constituents.Samples from Heilongjiang and Inner Mongolia gen-erally showed higher contents of all three compounds than those from Hebei,Shaanxi,Gansu,and Henan.The content ranges were 2.09-6.12 mg/g for prim-O-glucosylcimifugin,1.37-6.12 mg/g for cimifugin,and 1.40-3.74 mg/g for 5-O-methylvisammioside.PCA and HCA classified all samples into three clusters,with the Northeastern origin forming a separate high-content group.Significant differences(P<0.05)were found between wild and cultivated samples.GRA indicated that some samples from Gansu had a more bal-anced proportion of constituents and thus higher comprehensive quality relational degrees.Conclusion The chromone constituents in Radix Saposhnikoviae exhibit stable geographic distribution patterns,providing chemical evidence for the superior quality of the tradi-tional"Guan Fangfeng(one variety from Northeast China)"and supporting origin traceability,quality standard establishment,and culti-var selection.关键词
防风/灰色关联度分析法/主成分分析(PCA)/质量差异分析/色原酮类Key words
Radix Saposhnikoviae/Grey relational analysis/Principal component analysis/Quality difference analysis/Chro-mones分类
医药卫生引用本文复制引用
姬海月,张凌霄,王二欢,朱佩,何志鹏,杨佳豪,王思蕴,胡本祥..基于HPLC多成分定量与化学模式识别的不同产地防风质量差异分析[J].时珍国医国药,2026,37(15):2857-2862,6.基金项目
国家自然科学基金(82204578) （82204578）
陕西国际商贸学院校级课题项目(SMXY202466) （SMXY202466）
陕西省一般项目(2025SF-YBXM-267) （2025SF-YBXM-267）
陕西省科技厅项目(2023-YBSF-036) （2023-YBSF-036）
