现代中西医结合杂志2026，Vol.35Issue(12)：1607-1614,1625,9.DOI:10.3969/j.issn.1008-8849.2026.12.001
清肝健脾活血方对CCl4诱导肝纤维化大鼠的保护作用研究
Prescription for clearing liver,fortifying spleen and resolving stasis alleviates liver injury in rats with CCl4-induced liver fibrosis
摘要
Abstract
Objective It is to observe the hepatoprotective effect of prescription for clearing liver,fortifying spleen and resolving stasis(PCLFSRS)on hepatocytes in rats with liver fibrosis induced by CCl4.Methods A total of 72 male SD rats were randomly divided into normal group,model group,high-dose,medium-dose and low-dose groups of PCLFSRS,Biejia-jian Pill(BJJP)group,with 6 rats in each group.The rats of all the groups except for the normal group were prepared with intraperitoneal injection of 40%CCl4 olive oil suspension to establish models of liver fibrosis.Starting from the 2rd day of modeling,high-dose,medium-dose and low-dose groups of PCLFSRS were administered PCLFSRS 32.084 g/kg,16.042 g/kg,and 8.021 g/kg via gavage,respectively,while the BJJP group was administered BJJP 0.9255 g/kg via gavage,the normal group and model group were given pure water via gavage,all once daily,continuously treated for 8 weeks.The pathological morphology of liver tissue was observed by HE staining,Masson staining,and Sirius red staining.The serum levels of ala-nine aminotransferase(ALT),aspartate aminotransferase(AST),albumin(ALB),lactate dehydrogenase(LDH),total bilirubin(TBil),hyaluronic acid(HA),laminin(LN),type Ⅲ procollagen(PC-Ⅲ),and type IV collagen(Ⅳ-C),as well as the contents of hydroxyproline(Hyp),glutathione peroxidase(GPX),and glutathione S-transferase(GST)in liver tissue were measured by the kit methods,the apoptosis of hepatocytes was assessed by TUNEL method,and the protein ex-pressions of Bax and Caspase-3 in liver tissue were detected by Western blot.Results Compared with the normal group,in-flammatory cell aggregation with fibrous septa formation in liver tissue were found in the rats of the model group,the serum levels of AST/ALT,LDH,TBil,HA,LN,PC-Ⅲ and Ⅳ-C,as well as then contents of Hyp and GST in liver tissue were significantly increased(all P<0.05),while the serum level of ALB and content of GPX in liver tissue were significantly decreased(all P<0.05),the apoptosis rate and relative protein expressions of Bax and Caspase-3 in liver tissue were sig-nificantly increased(all P<0.05).Compared with the model group,the fibrosis of liver tissue in the rats of the hight-dose group of PCLFSRS and BJJP group were obviously alleviated,the serum levels of AST/ALT,LDH,TBil,HA,LN,PC-Ⅲand Ⅳ-C,as well as then contents of Hyp and GST in liver tissue were significantly decreased(all P<0.05),while the contents of GPX in liver tissue were significantly decreased(all P<0.05),the apoptosis rates and relative protein expres-sions of Bax and Caspase-3 in liver tissue were significantly decreased(all P<0.05),the serum level of ALB was signifi-cantly increased,while the relative protein expression of Bax in liver tissue was significantly decreased in the high-dose group of PCLFSRS.Conclusion PCLFSRS can significantly alleviate CCl4 induced liver injury in rats,reduce liver cell ap-optosis,and protect liver cells.关键词
清肝健脾活血法/四氯化碳/肝纤维化/细胞凋亡Key words
clearing liver,fortifying spleen and resolving stasis/CCl4/liver fibrosis/apoptosis分类
医药卫生引用本文复制引用
刘定,潘富珍,曹洪欣,符小聪,杨沈秋,张禹..清肝健脾活血方对CCl4诱导肝纤维化大鼠的保护作用研究[J].现代中西医结合杂志,2026,35(12):1607-1614,1625,9.基金项目
江门市基础与应用基础研究重点项目(2220002000220) （2220002000220）
黑龙江省自然科学基金联合引导项目(LH2019H109) （LH2019H109）
