现代中西医结合杂志2026，Vol.35Issue(12)：1615-1625,11.DOI:10.3969/j.issn.1008-8849.2026.12.002
健脾消渴方干预2型糖尿病的物质基础及潜在作用机制研究
Molecular basis and potential mechanisms of action of wasting-thirst decoction for fortifying spleen in the treatment of type 2 diabetes
摘要
Abstract
Objective It is to explore the potential mechanism of wasting-thirst decoction for fortifying spleen(WT-DFS)in the treatment of type 2 diabetes(T2DM)by network pharmacology,molecular docking and untargeted metabolo-mics.Methods ①The effective chemical components of WTDFS were mined from BATMAN-TCM database;the T2DM tar-gets and their common targets shared with WTDFS were screened out from GeneCards database;the Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were performed on OmicShare plat-form;a protein-protein interaction(PPI)network was constructed on STRING platform and the core targets were analyzed by Cytoscape topological parameters;the batch molecular docking was performed through AutoDock vina.②Eighty male SD rats were selected,20 of which were randomly assigned to the normal group,and the remaining rats were prepared to estab-lish models of T2DM.The successfully modeled rats were randomly divided into model group,WTDFS group and metformin group,with 20 rats in each group.The WTDFS group was given WTDFS 20 g/kg via gavage,the metformin group was giv-en metformin 200 mg/kg by gavage,and the normal group and model group were given an equal volume of distilled water via gavage,all once daily,continuously treated for 4 weeks.The differentially expressed metabolites in the serum of rats in each group were detected by non-targeted metabolomics techniques,and bioinformatics data mining was performed.Results①There were 95 effective ingredients in WTDFS,corresponding to 407 targets,and 3024 targets corresponding to T2DM,a total of 190 common targets were shared between T2DM and WTDFS;the GO and KEGG enrichment results showed that they were mostly concentrated in metabolic field;PPI network and topological analysis showed that the core targets were NOS1,CACNG2,PRKCA,CACNA1C and DLG4;molecular docking results indicated that the main active ingredients had good binding strength with the core targets.②The main differentially expressed metabolites in the serum of rats in the mod-el group and WTDFS group were Phloretin 2'-O-glucuronide,Paeonol,etc.,which were closely related to signaling path-ways such as bile secretion signaling pathway,glucagon signaling pathway,cAMP signaling pathway.Conclusion WTDFS plays a therapeutic effect in the treatment of T2DM via regulating signal pathways such as cAMP pathway.关键词
健脾消渴方/2型糖尿病/信号通路/网络药理学/非靶向代谢组学Key words
wasting-thirst decoction for fortifying spleen/type 2 diabetes mellitus/signal pathway/network pharma-cology/untargeted metabolomics分类
医药卫生引用本文复制引用
邓龙飞,高柳,孙婷,储莺,顾晶晶,姚静雯,王骏..健脾消渴方干预2型糖尿病的物质基础及潜在作用机制研究[J].现代中西医结合杂志,2026,35(12):1615-1625,11.基金项目
上海市卫生健康委员会中医药科研项目(2022QN075) （2022QN075）
浦东新区"国家中医药传承创新发展试验区"建设项目(PDZY-2026-0215) （PDZY-2026-0215）
