现代中西医结合杂志2026，Vol.35Issue(12)：1632-1636,1643,6.DOI:10.3969/j.issn.1008-8849.2026.12.004
丹瓜方调控SIRT1/PGC-1α通路对2型糖尿病大鼠肝脏损伤的影响研究
Dangua decoction ameliorates hepatic injury in rats with type 2 diabetes mellitus via regulating the SIRT1/PGC-1α pathway
摘要
Abstract
Objective It is to explore the regulatory effects of Dangua decoction(DGD)on hepatic injury and the silent information regulator 1(SIRT1)/peroxisome proliferator-activated receptor gamma coactivator 1α(PGC-1α)pathway in the rats with type 2 diabetes mellitus(T2DM).Methods Twenty male SD rats were selected,5 of which were randomly as-signed to the control group,while the remaining rats were fed with a high-sugar,high-fat diet and given 1%streptozotocin solution via intraperitoneal injection to establish T2DM modes.The successfully modeled rats were randomly divided into model group,DGD group and metformin group,with 5 rats in each group.The normal group and model group were admin-istered sterile water at a dose of 10 mL/kg via gavage,the DGD group was administered DGD solution at a dose of 20 g/kg via gavage,and the metformin group was administered metformin solution at a dose of 0.11 g/kg via gavage,all once daily,continuously treated for 14weeks.The levels of fasting plasma glucose(FPG),2 h postprandial glucose(2hPG),glycated hemoglobin(HbA1c),total cholesterol(TC),triglycerides(TG),malondialdehyde(MDA)and interleukin-6(IL-6)in serum of rats of each group were measured,the liver histopathology was observed by HE staining and Masson's staining,and the protein expressions of SIRT1 and PGC-1α in liver tissue were decocted by Western blot.Results Compared with the control group,the levels of FPG,2hPG,HbA1c,TC,TG,MDA and IL-6 in serum of rats in the model group were signifi-cantly increased(all P<0.05);the liver lobule structure was disrupted with extensive inflammatory cell infiltration and in-creased collagen fibers;the relative protein expressions of SIRT1 and PGC-1α in liver tissue were significantly reduced(all P<0.05).Compared with the model group,the levels of FPG,2hPG,HbA1c,TC,TG,MDA and IL-6 in serum of rats in the DGD group and metformin group were significantly decreased(all P<0.05);the liver histopathological injury was ameliorated;the relative protein expressions of SIRT1 and PGC-1α in liver tissue were significantly increased(all P<0.05).Conclusion DGD may ameliorate hepatic injury in T2DM rats via regulating the hepatic SIRT1/PGC-1α pathway,thereby reducing oxidative stress and inflammatory responses.关键词
丹瓜方/2型糖尿病/沉默信息调节因子1/过氧化物酶体增殖物激活受体γ共激活因子1α/肝脏损伤Key words
Dangua decoction/type 2 diabetes mellitus/SIRT1/PGC-1α/hepatic injury分类
医药卫生引用本文复制引用
林妍彦,王志塔,韩壮,陈奇炜,靳霖溪,洪鑫淼,衡先培,杨柳清,李亮,何卫东..丹瓜方调控SIRT1/PGC-1α通路对2型糖尿病大鼠肝脏损伤的影响研究[J].现代中西医结合杂志,2026,35(12):1632-1636,1643,6.基金项目
国家自然科学基金资助项目(82574894,82274389,82205071) （82574894,82274389,82205071）
福建省科技创新联合资助项目(2025Y9560,2025Y9571) （2025Y9560,2025Y9571）
