现代中西医结合杂志2026，Vol.35Issue(12)：1637-1643,7.DOI:10.3969/j.issn.1008-8849.2026.12.005
基于TLR4/NLRP3通路探讨消炎散对急性胰腺炎的影响及作用机制
Xiaoyansan alleviates inflammatory damage in acute pancreatitis via inhibiting the activation of the TLR4/NLRP3 pathway
摘要
Abstract
Objective It is to explore the therapeutic effect of Xiaoyansan on mice with acute pancreatitis(AP),and to explore its mechanism of action.Methods Forty male C57BL/6J mice were randomly divided into control group,AP group,low-dose,medium-dose and high-dose groups of Xiaoyansan,with 8 mice in each group.The mice of all groups except for the control group were intraperitoneally injected with caerulein to establish AP models.After modeling,the low-dose,medi-um-dose and high-dose groups of Xiaoyansan were given Xiaoyansan 1,2,4 g/kg via gavage,respectively,all once daily,continuously treated for 7 days.The ascites volume of mice in each group was measured,and the levels of amylase(AMY)and lipase(LPS)in serum of mice in each group were measured by automatic biochemical analyzer,the levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and interleukin-18(IL-18)in serum were measured by ELISA,the pathological changes of pancreatic tissues of mice in each group were observed by HE staining,the positive expressions of Toll-like receptor 4(TLR4)and NLR family pyrin domain protein 3(NLRP3)in pancreatic tissues of mice in each group were detected by immunohistochemical staining,the expressions of TLR4/NLRP3 pathway-related proteins TLR4,myeloid differentiation factor 88(MyD88),phosphorylated nuclear factor-κB(p-NF-κB),nuclear factor-κB(NF-κB),NLRP3,and cysteine protease-1(Caspase-1)in pancreatic tissues of mice in each group were detected by Western blot.Results Compared with the control group,the ascites volume and the levels of AMY,LPS,TNF-α,IL-6 and IL-18 in serum of mice in the AP group were significantly increased(all P<0.05);the pancreatic tissue structure was destroyed with wid-ened interstitial tissue,degenerated acinar cells and infiltration of a large number of inflammatory cells;the mean optical density values of expressions of TLR4 and NLRP3 in pancreatic tissue,as well as the relative protein expressions of TLR4,MyD88,p-NF-κB,NLRP3 and Caspase-1 were all significantly increased(all P<0.05).Compared with the AP group,the ascites volume and the levels of AMY,LPS,TNF-α,IL-6 and IL-18 in serum of mice in each dose groups of Xiaoyan-san were significantly decreased(all P<0.05);the pathological lesions and inflammatory cell infiltration in pancreatic tis-sue were alleviated;the mean optical density values of expressions of TLR4 and NLRP3 in pancreatic tissue,as well as the relative protein expressions of TLR4,NLRP3 and Caspase-1 were all significantly decreased(all P<0.05),furthermore,the relative protein expressions of MyD88 and p-NF-κB were also significantly increased in the medium-dose and high-dose groups of Xiaoyansan.Conclusion Xiaoyansan can inflammatory damage in acute pancreatitis via inhibiting the activation of the TLR4/NLRP3 pathway.关键词
急性胰腺炎/消炎散/Toll样受体4/NOD样受体热蛋白结构域相关蛋白3/炎症Key words
acute pancreatitis/Xiaoyansan/Toll-like receptor 4/NLR family pyrin domain-containing protein 3/in-flammation分类
医药卫生引用本文复制引用
焦敏,袁贺霞,康莹莹,阎新燕,于鲁海,李红健,吴建华..基于TLR4/NLRP3通路探讨消炎散对急性胰腺炎的影响及作用机制[J].现代中西医结合杂志,2026,35(12):1637-1643,7.基金项目
新疆维吾尔自治区卫生健康青年医学科技人才专项科研项目(WJWY-202458) （WJWY-202458）
