云南师范大学学报（自然科学版）2026，Vol.46Issue(3)：33-40,8.DOI:10.7699/j.ynnu.ns-2026-33
快速滤过型净化结合超高效液相色谱—串联质谱法同时测定水产品中10种药物残留量
Simultaneous Determination of 10 Drug Residues in Aquatic Products Using Multi-plug Filtration Cleanup Coupled with Ultra-high Performance Liquid Chromatography-tandem Mass Spectrometry
杜鑫 1易珊珊 1陈伟成 1覃明丽1
作者信息
- 1. 南充农产品质量监测检验中心,四川南充 637000
- 折叠
摘要
Abstract
An analytical method based on multi-plug filtration cleanup(m-PFC)coupled with ultra-high performance liquid chromatography-tandem mass spectrometry was developed for the simultaneous de-termination of 10 drug residues,including quinolones,chloramphenicol and diazepam,in aquatic prod-ucts.Samples were extracted with 1％formic acid in acetonitrile after pretreatment with ammonium acetate solution,followed by high-speed freezing centrifugation and m-PFC purification.Detection was performed using electrospray ionization in positive and negative modes with multiple reaction monito-ring,and quantification was based on matrix-matched calibration.The method showed good linearity(r＞0.999)in the range of 0.5～50 μg·L-1,with a limit of quantification of 1 μg·kg-1and detection limits of 0.2～0.4 μg·kg-1.At three spiked levels(1,10,and 100 μg·kg-1),the average recoveries ranged from 76.2％to 108.4％,with relative standard deviations of 2.6％～6.8％.The proposed methodis simple,sensitive,accurate and suitable for high-throughput rapid detection of multi-drug residues in aquatic products.关键词
快速滤过型净化/喹诺酮类/地西泮/氯霉素/水产品Key words
Multi-plug filtration cleanup(m-PFC)/Quinolones/Diazepam/Chloramphenicol/Aquatic products分类
化学化工引用本文复制引用
杜鑫,易珊珊,陈伟成,覃明丽..快速滤过型净化结合超高效液相色谱—串联质谱法同时测定水产品中10种药物残留量[J].云南师范大学学报（自然科学版）,2026,46(3):33-40,8.
