医学新知2026，Vol.36Issue(7)：763-770,8.DOI:10.12173/j.issn.1004-5511.202606001
OSM/OSMR信号促进胶质母细胞瘤细胞侵袭的作用与机制研究
The effects and mechanism of OSM/OSMR signaling in promoting glioblastoma cell invasion
摘要
Abstract
Objective To investigate the effects and mechanism of oncostatin M(OSM)/oncostatin M receptor(OSMR)signaling on the invasive ability of glioblastoma(GBM)cells.Methods Public single-cell sequencing datasets were analyzed to clarify the cellular localization of OSM and OSMR in GBM,and verified by immunofluorescence double staining in clinical samples.Transwell invasion assays were performed to evaluate the effects of OSM treatment and OSMR knockdown on the invasive ability of LN229 cells.Transcriptome sequencing combined with bioinformatics analysis was used to screen differentially expressed genes and enrichment pathways.Western blot was applied to verify the activation of the PI3K/Akt pathway and the expression changes of proteins associated with proneural-mesenchymal transition(PMT).Results Single-cell sequencing and immunofluorescence results showed that OSM was mainly expressed in tumor-associated macrophages,while OSMR was predominantly expressed in GBM cells.OSM treatment significantly enhanced the invasive ability of GBM cells,whereas OSMR knockdown markedly inhibited cell invasion.Transcriptome sequencing and enrichment analysis revealed that differentially expressed genes were significantly enriched in the PI3K/Akt pathway,and Western blot confirmed that OSM/OSMR signaling activated the PI3K/Akt pathway.OSMR knockdown upregulated the expression of the proneural marker OLIG2 and downregulated the expression of the mesenchymal marker CD44,thereby suppressing PMT in GBM cells.Conclusion OSM/OSMR signaling promotes GBM cell invasion by activating the PI3K/Akt pathway and inducing PMT.This signaling axis is expected to become a potential therapeutic target for GBM.关键词
胶质母细胞瘤/抑瘤素M/抑瘤素M受体/肿瘤相关巨噬细胞/侵袭/PI3K/Akt通路/前神经元-间充质转化Key words
Glioblastoma/Oncostatin M/Oncostatin M receptor/Tumor-associated macrophages/Invasion/PI3K/Akt pathway/Proneural-mesenchymal transition分类
医药卫生引用本文复制引用
肖靖芳,刘庆,张军鸽,王帅,颜泽萱,吉佳乐,孔维凯,梁媚,曹棉富..OSM/OSMR信号促进胶质母细胞瘤细胞侵袭的作用与机制研究[J].医学新知,2026,36(7):763-770,8.基金项目
国家自然科学基金青年科学基金项目(82103590) （82103590）
