养殖与饲料2026，Vol.25Issue(8)：8-12,5.DOI:10.13300/j.cnki.cn42-1648/s.2026.08.002
健康鲫肠道菌群结构及转录组特征分析
Bacterial community structure and transcriptomic characteristics of the intestine in healthy crucian carp(Carassius auratus)
摘要
Abstract
[Objectives]The expression profiles of intestinal genes and the structure of intestinal microbiota in healthy crucian carp(Carassius auratus)were analyzed to provide a reference for the in-depth elucidation of intestinal homeostasis and the immune mechanisms underlying the resistance to diseases in crucian carp.[Meth-ods]From a population of healthy crucian carp(mean weight 100±5 g,body length 15±2 cm),9 individuals were randomly selected.Intestinal tissues were pooled and homogenized after anesthesia and sterile dissection.The mixed samples were used for Illumina NovaSeq platform and 16S rRNA high-throughput sequencing to analyze the intestinal microbiota structure and transcriptome.[Results]The intestinal microbiota of crucian carp was dominated by Proteobacteria and Cetobacterium.The results of analyzing alpha(α)diversity and beta(β)diversity showed that the richness and evenness of the microbiota were high,with Simpson index higher than 0.97.A total of 62 547 genes transcripts were obtained.The results of analyzing the Gene Ontology(GO)en-richment showed that highly expressed genes were mainly involved in biological processes including gene translation,innate immunity and the transport of iron ions.The results of analyzing the Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment showed that these genes were predominantly enriched in energy me-tabolism,protein processing(folding,sorting and degradation),cell growth and death,as well as immune-related signaling pathways such as TLRs,NLRs and NF-κB.[Conclusions]Proteobacteria and Cetobacterium serve as the dominant intestinal microbiota in healthy crucian carp.Intestinal genes are mainly involved in bio-logical functions including energy metabolism,protein metabolism,and immune defense,and are enriched in innate immune signaling pathways including TLRs,NLRs,NF-κB,which regulate the growth,disease resis-tance and immune mechanisms in crucian carp.关键词
鲫/肠道菌群/转录组/先天免疫/肠道稳态/高通量测序Key words
crucian carp(Carassius auratus)/intestinal microbiota/transcriptome/innate immunity/intes-tinal homeostasis/high-throughput sequencing引用本文复制引用
林轩,张杨,胡秀彩,李彧,张文雅,管振国,吕爱军..健康鲫肠道菌群结构及转录组特征分析[J].养殖与饲料,2026,25(8):8-12,5.基金项目
天津市大学生创新训练计划项目(202510061207) （202510061207）
津甘双地科技特派员项目(24CXNA078) （24CXNA078）
