中草药2026，Vol.57Issue(15)：5848-5858,11.DOI:10.7501/j.issn.0253-2670.2026.15.007
柴胡皂苷D-大麻二酚自组装纳米粒的制备及细胞药效学评价
Preparation and cytopharmacology evaluation of self-assembled saikosaponin D-cannabidiol nanoparticles
摘要
Abstract
Objective To prepare a self-assembled nanoparticles of saikosaponin D(SSD)and cannabidiol(CBD)and preliminarily investigate their anti-hepatoma mechanism in vitro.Methods The SSD-CBD self-assembled nanoparticles were constructed using the nano co-precipitation method at a mass ratio of 3∶1,and their assembly mechanisms were characterized by techniques including X-ray photoelectron spectroscopy(XPS),Fourier transform infrared spectroscopy(FTIR),and 1H-1H nuclear overhauser enhancement spectroscopy(1H-1H NOESY).Its physicochemical properties were evaluated by transmission electron microscopy(TEM)and dynamic light scattering(DLS).The antitumor activity,apoptosis induction,and preliminary mechanisms were systematically assessed by MTT assay,flow cytometry(Annexin V-FITC/PI staining),and cysteinyl aspartate specific proteinase(Caspase)activity assays.Results The assembly was self-assembled through hydrogen bonding and hydrophobic interactions,with SSD and CBD showing a synergistic effect against HepG2 cells(CI=0.79).It exhibited pH-responsive drug release,specifically releasing drugs in the tumor microenvironment(pH 6.8)while remaining stable at normal physiological environment(pH 7.4).Mechanistic studies indicated that it primarily induced HepG2 cell death by activating the mitochondrial apoptotic pathway.Conclusion The constructed SSD-CBD self-assembled nanoparticles offer a carrier-free strategy that can simultaneously improve the delivery efficiency of CBD and achieve precise collaborative drug delivery,providing an experimental basis for the development of intelligent nanomedicine based on SSD.关键词
柴胡皂苷D/大麻二酚/细胞凋亡/自组装纳米粒/细胞药效学Key words
saikosaponin D/cannabidiol/apoptosis/self-assembled nanoparticles/cytopharmacology分类
医药卫生引用本文复制引用
俞泽如,张怡,于忆骅,孙琦,宋国虎,余马,陈华,辛超..柴胡皂苷D-大麻二酚自组装纳米粒的制备及细胞药效学评价[J].中草药,2026,57(15):5848-5858,11.基金项目
西藏自治区重点研发计划(XZ202401ZY0020) （XZ202401ZY0020）
药用植物种质资源库建设(2021-I2M-1-032) （2021-I2M-1-032）
国家岗位产业体系专家项目(CARS-21) （CARS-21）
四川省区域创新合作项目(2026YFHZ0163) （2026YFHZ0163）
