中草药2026，Vol.57Issue(15)：5874-5889,16.DOI:10.7501/j.issn.0253-2670.2026.15.009
负载姜黄素可打印氨基酸基低共熔凝胶敷料制备及其体外性能和治疗口腔溃疡药效作用研究
Preparation,in vitro performance,and therapeutic efficacy against oral ulcers of curcumin-loaded printable amino acid-based deep eutectic gel dressings
摘要
Abstract
Objective To prepare an amino acid-based deep eutectic gel dressing and study in vitro performance and the in vivo therapeutic effect on oral ulcer.Methods Deep eutectic solvents(DESs)were screened using lysine,proline,arginine,and glycine as hydrogen bond donors,and lactic acid,citric acid,glycerol,mannitol,and malic acid as acceptors,with curcumin solubility identifying the optimal system.Gel dressings were prepared by optimizing the formulation process based on the composite matrix of gelatin methacryloyl/chitosan methacryloyl(GelMA/CSMA)in combination with 3D printing technology.Its mechanical properties,adhesiveness,swelling,and biocompatibility were evaluated,and in vivo efficacy was verified in an SD rat oral ulcer model.Results The optimal DES system(proline-lactic acid,1∶12)increased curcumin solubility over 700-fold(0.789 mg/mL)compared to its solubility in water.The optimal printing formulation and process parameters were as follows:1％CSMA,5％GelMA,0.1％lithium phenyl-2,4,6-trimethylbenzoylphosphinate(LAP),0.02％tartrazine,20％DES content,100 μm layer height,and a layer-by-layer exposure time of 25 s.The obtained amino acid-based gel dressing had excellent mechanical properties,with a 2.5 N adhesion force at 25 min and an approximately 500％swelling ratio at 6 h.In vitro biocompatibility showed that the survival rate of L929 cells was all above 90％,and the DES system had no significant cytotoxicity.It also showed high in vitro biocompatibility(L929 viability＞90％,non-cytotoxic).In vivo,the gel achieved localized,sustained curcumin release in SD oral ulcer model rats,reducing ulcer area by 40％with decreased neutrophil density and inflammatory infiltration.Conclusion Successfully prepared an amino acid-based DES gel dressing loaded with curcumin,which exhibits excellent wound adaptability and precise drug release behavior,offering an efficient and safe therapeutic strategy for oral ulcers.关键词
低共熔溶剂/氨基酸/姜黄素/凝胶敷料/口腔溃疡Key words
deep eutectic solvents/amino acids/curcumin/gel dressings/oral ulcer分类
医药卫生引用本文复制引用
关晓朵,严文政,李惠杰,杨根生,杨庆良,贺悦..负载姜黄素可打印氨基酸基低共熔凝胶敷料制备及其体外性能和治疗口腔溃疡药效作用研究[J].中草药,2026,57(15):5874-5889,16.基金项目
湖州市科技局公益研究项目—人口健康类(2025GY121) （2025GY121）
浙江省中医药科技计划项目资助(2025ZL027) （2025ZL027）
