中草药2026，Vol.57Issue(15)：5890-5901,12.DOI:10.7501/j.issn.0253-2670.2026.15.010
基于HPLC指纹图谱、Q-TOF-MS/MS和模式识别定性与多成分定量相结合的金荞麦片质量评价
Quality evaluation of Fagopyri Dibotryis Rhizoma Tablets based on qualitative analysis of HPLC fingerprint,Q-TOF-MS/MS and pattern recognition qualitative analysis combined with quantitative analysis of multi-constituents
摘要
Abstract
Objective To evaluate the quality of Fagopyri Dibotryis Rhizoma Tablets(FDRT)produced by different companies.Methods A total of 18 batches of FDRT produced by company A and 20 batches of FDRT produced by company B were collected,respectively.The fingerprints of 38 batches FDRT were determined using HPLC,the common peaks were assigned and the constituents of these peaks were identified by quadrupole time-of-flight mass spectrometry(Q-TOF-MS/MS).Using quantitative common peak areas as variables,pattern recognition analysis of the fingerprint was conducted using hierarchical cluster analysis(HCA),principal component analysis(PCA),and orthogonal partial least squares discriminant analysis(OPLS-DA).The content of the nine constituents confirmed by comparison with reference substances and have good separation in samples were determined.Results There were 33 common peaks in the fingerprint of 38 batches of FDRT,and a total of 53 constituents were identified by Q-TOF-MS/MS,including 21 tannins,12 phenolics,seven flavonoids,seven phenylpropanoid glycosides,two amino acids,two organic acids,one alkaloid,and one terpenoid,among them,three phenolics(gallic acid,protocatechuic acid,and protocatechualdehyde),five tannins(procyanidin B1,B2,B3,C1,C2),four flavonoids(catechin,epicatechin,epicatechin gallate,and rutin)were confirmed by reference substance.The separation degree of nine constituents except for procyanidin C2,B2,and rutin was relatively good.The average content of these nine constituents in FDRT produced by company B was significantly higher than that in FDRT produced by company A(P＜0.01).The results of HCA,PCA,and OPLS-DA were consistent,clearly distinguished the FDRT produced by company A and company B into two categories.The constituents corresponding to common peaks 30,29,16,17,2,21,10,11,18,20,and 27,such as procyanidin C2,B2,C1,B1,B3,gallic acid,epicatechin,etc.,are the marker constituents responsible for the quality differences in FDRT produced by two enterprises.Conclusion There is a significant quality difference in FDRT produced by two companies.分类
医药卫生引用本文复制引用
曹溢,何梦,戴莹,李余佳,杨军炎,窦志华..基于HPLC指纹图谱、Q-TOF-MS/MS和模式识别定性与多成分定量相结合的金荞麦片质量评价[J].中草药,2026,57(15):5890-5901,12.基金项目
江苏省南通市科技计划社会民生重点项目(MS12022012) （MS12022012）
江苏省药学会正大天晴医院药学科研基金重点项目(Q202205) （Q202205）
江苏省南通市卫生健康委员会科研课题(WSZ2022034) （WSZ2022034）
南通市药学会常州四药医院药学科研基金项目(ntyx2201) （ntyx2201）
