中国实验动物学报2026，Vol.34Issue(7)：972-983,12.DOI:10.3969/j.issn.1005-4847.2026.07.004
利用小鼠原代软骨细胞体外过表达模型探究核糖体蛋白S29在骨关节炎中的作用
Exploring the role of ribosomal protein S29 in osteoarthritis using an in vitro mouse primary chondrocyte overexpression model
摘要
Abstract
Objective To investigate the role of ribosomal protein S29(Rps29)in osteoarthritis using an in vitro Rps29-overexpressing mouse primary chondrocyte model.Methods Rps29 expression in cartilage of meniscectomy(DMM)-induced osteoarthritis(OA)mice was detected by immunohistochemical staining.Through primary mouse chondrocyte isolation and culture,an Rps29-overexpressing model was established through cell transfection with control and Rps29-overexpressing vectors carrying the green fluorescence reporter gene.Expression of Rps29 mRNA and protein in the Rps29-overexpressing cell model was examined with reverse-transcription fluorescence quantitative PCR(RT-qPCR)and Western Blot.EdU incorporation was used to analyze Rps29-overexpressing chondrocyte proliferation.Immunofluorescence,RT-qPCR,and Western Blot were also utilized to measure expression levels of key molecules in OA,such as Collagen Ⅱ,Collagen Ⅹ,tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),nuclear factor-κB(NF-κB),and matrix metalloproteinase-13(MMP13).Results Rps29 expression in the knee joint of the DMM group was increased compared with the control group.Rps29 mRNA and protein expression were both significantly increased in Rps29-overexpressing primary chondrocytes compared with the control group.Decreased proliferation,decreased Collagen Ⅱ expression,and increased Collagen Ⅹ expression were detected in Rps29-overexpressing chondrocytes,andTNF-α,IL-6,and NF-κB expression was also significantly increased.Conclusions The molecular pathological changes of Rps29-overexpressing primary chondrocytes resembled an OA phenotype.Rps29 promoted OA by activating inflammatory responses and NF-κB signaling,providing new clues and a preliminary theoretical basis for understanding the role of Rps29 in OA.关键词
核糖体蛋白S29/骨关节炎/小鼠原代软骨细胞/过表达/体外细胞模型Key words
ribosomal protein S29/osteoarthritis/primary mouse chondrocytes/overexpression/in vitro cell models分类
生物科学引用本文复制引用
唐玉玲,李斯特,钱洪安,王春芳,李子璇,崔晶,黄婧姝,邱业峰,王进..利用小鼠原代软骨细胞体外过表达模型探究核糖体蛋白S29在骨关节炎中的作用[J].中国实验动物学报,2026,34(7):972-983,12.基金项目
军队实验动物专项科研课题项目(SYDW[2018]02 号),军事医学研究院青年人才培育基金项目(AMMS-QNPY-2021-019). Funded by Military Experimental Animal Special Research Project(SYDW[2018]02),Youth Talent Cultivation Found Project of Military Medical Research Institute(AMMS-QNPY-2021-019). （SYDW[2018]02 号）
