中国实验动物学报2026，Vol.34Issue(7)：984-997,14.DOI:10.3969/j.issn.1005-4847.2026.07.005
哈它各其-7联合胡麻油通过调控炎症反应与GSK3β信号通路促进压疮愈合的研究
Hatagaqi-7 combined with sesame oil promotes pressure-ulcer healing via inflammation modulation and GSK3β signaling
摘要
Abstract
Objective This study aimed to explore the potential mechanisms of the Mongolian medicine hatagaqi-7 combined with sesame oil in promoting pressure ulcer wound healing based on network pharmacology,and to verify the findings through molecular docking and animal experiments.Methods Potential active components and targets of hatagaqi-7 combined with sesame oil,as well as pressure ulcer-related targets,were identified through database and literature searches.The common targets were used to construct a protein-protein interaction(PPI)network,followed by gene ontology(GO)and kyoto encyclopedia of genes and genomes(KEGG)pathway analyses,and molecular docking.A rat model of pressure ulcer was established and divided into six groups:control(K),model(M),hatagaqi-7(Q),sesame oil(H),hatagaqi-7 combination sesame oil(H+Q)and yunnan baiyao(Y)groups(n=10).All groups received corresponding treatments for 14 d.Wound morphology,healing rate,histopathological changes,and the expression of interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),glycogen synthase kinase-3β(GSK3β),NF-κB p65 and β-catenin were evaluated.Results Network pharmacology identified 35 active compounds,644 drug targets,and 398 disease targets.Core targets included BCL2,TNF-α,NFKB1,IL-6,IL-1β,and GSK3β,mainly involving the TNF inflammatory response,and Wnt signaling pathways.Molecular docking indicated that the major active components-caffeine and estradiol-exhibited strong binding affinities with GSK3β,TNF-α,IL-1β,and IL-6.Animal experiments showed that the(H+Q)group had the highest wound healing rate and the most abundant collagen deposition.The expression levels of IL-1β,IL-6,TNF-α,GSK3β,and NF-κB p65 were significantly downregulated in the(H+Q)group(P＜0.05),with greater reductions compared with the H,Q,Y groups(P＜0.05).The expression of β-catenin was significantly upregulated in the(H+Q)group(P＜0.05),and the magnitude of upregulation was significantly greater than that in the H,Q,Y groups(P＜0.05).Conclusions Hatagaqi-7 combined with sesame oil significantly promotes pressure ulcer healing,potentially through suppressing inflammatory responses and regulating the GSK3β signaling pathway.The combination therapy demonstrates potential clinical value and provides an experimental basis for the application of mongolian medicine in pressure ulcer treatment.关键词
哈它各其-7/压疮/网络药理学/分子对接/GSK3βKey words
hatagaqi-7/pressure ulcer/network pharmacology/molecular docking/GSK3β分类
生物科学引用本文复制引用
张心雨,侯婧,杨一枭,魏林林,吴思元,宋鹤天,张志芳,刘春燕..哈它各其-7联合胡麻油通过调控炎症反应与GSK3β信号通路促进压疮愈合的研究[J].中国实验动物学报,2026,34(7):984-997,14.基金项目
内蒙古医学科学院公立医院科研联合基金科技项目(2023GLLH0376). Funded by Science and Technology Program of the Joint Fund of Scientific Research for the Public Hospitals of Inner Mongolia Academy of Medical Sciences(2023GLLH0376). （2023GLLH0376）
