中国实验动物学报2026，Vol.34Issue(7)：998-1005,8.DOI:10.3969/j.issn.1005-4847.2026.07.006
幼年大鼠LED光源性近视模型的建立
Establishment of a myopia model in juvenile rats using light-emitting diodes
摘要
Abstract
Objective To establish and evaluate a rat model of myopia induced by light-emitting diode(LED)light exposure.Methods Forty 3-week-old male SPF rats were randomly divided into control and model groups(n=20).Axial length was measured before the experiment and at weeks 2,4,and 6.Scleral morphological changes were observed using hematoxylin and eosin(HE)staining.Serum melatonin concentration was measured by enzyme-linked immunosorbent assay.Western Blot was applied to determine matrix metalloproteinase-2(MMP-2)and tissue inhibitor of metalloproteinases-2(TIMP-2)protein expression in retinal tissue,and RT-qPCR was used to detect their messenger RNA(mRNA)expression in scleral tissue.Results The model group showed significantly elongated axial lengths at weeks 4 and 6 compared with the control group(P＜0.05).Scleral tissue exhibited disorganized and sparse collagen fibers,scleral layer thinning,and a significantly reduced serum melatonin concentration(P＜0.05).In addition,MMP-2 protein expression in retinal tissue and mRNA expression in scleral tissue were significantly upregulated,while TIMP-2 expression was significantly downregulated(P＜0.05).Conclusions Long-term LED light exposure can induce axial elongation,destroy scleral structural integrity,and regulate scleral MMP-2/TIMP-2 signaling,successfully establishing a rat model of LED light-induced myopia.关键词
近视/大鼠/LED光源/MMP-2/TIMP-2Key words
myopia/rat/LED light source/MMP-2/TIMP-2分类
生物科学引用本文复制引用
胡艺青,王艳,黄帅,洪滔,曹淑琼,郭佳,董德刚,刘志勇..幼年大鼠LED光源性近视模型的建立[J].中国实验动物学报,2026,34(7):998-1005,8.基金项目
江西中医药大学校级大学生创新创业项目(2024037,2024012),江西中医药大学校级科技创新团队发展计划(CXTD-22004). Funded by Jiangxi University of Chinese Medicine University-Level College Student Innovation and Entrepreneurship Training Program(2024037,2024012),University-Level Scientific and Technological Innovation Team Development Program of Jiangxi University of Chinese Medicine(CXTD-22004). （2024037,2024012）
