中国药房2026，Vol.37Issue(15)：1948-1953,6.DOI:10.6039/j.issn.1001-0408.2026.15.03
加味七方胃痛颗粒改善胃癌前病变的作用机制预测与验证
Prediction and verification of the mechanism of action of Jiawei qifang weitong granule in ameliorating precancerous lesions of gastric cancer
摘要
Abstract
OBJECTIVE To predict and verify the mechanism of Jiawei qifang weitong granule in ameliorating precancerous lesions of gastric cancer(PLGC).METHODS Transcriptomic data of PLGC-related were obtained from the GEO database to screen differentially expressed genes and conduct functional enrichment analysis.Targets related to gastric mucosal injury repair were screened via the GeneCards database.The active ingredient targets of Jiawei qifang weitong granule were predicted using the TCMSP and other databases.The intersection of differentially expressed genes,gastric mucosal repair targets and active ingredient targets was extracted.A protein-protein interaction network was constructed based on the STRING database,and the top 6 hub target genes with the highest connectivity were screened.A PLGC rat model was established by combining 1-methyl-3-nitro-1-nitrosoguanidine with alternating fasting and full feeding.Model rats were divided into model group,weifuchun group(positive control,0.40 g/kg),low-,medium-and high-dose Jiawei qifang weitong granule groups(9.73,19.44,38.90 g/kg),with 10 rats in each group.Another 10 unmodeled rats were set as control group.Rats in each group were intragastrically administered corresponding liquid medicine or normal saline once a day for 30 consecutive days.After the last administration,pathological morphological changes of rat gastric tissues were observed,and the mRNA expression levels of hub target genes as well as partial protein expression levels in gastric tissues were detected.RESULTS Transcriptomics and network pharmacology analyses revealed that the top 6 hub target genes ranked by connectivity included MMP9(matrix metalloproteinase 9),BCL2(B-cell lymphoma 2),IL10(interleukin 10),ICAM1(intercellular adhesion molecule 1),PTGS2(prostaglandin-endoperoxide synthase 2),and HIF1A(hypoxia-inducible factor 1α subunit).Animal experiments showed that compared with the model group,gastric mucosal pathological injury was significantly alleviated in medium-and high-dose Jiawei qifang weitong granule groups and weifuchun group.The pathological injury score of gastric mucosa,the mRNA expression levels of MMP9,PTGS2,ICAM1,BCL2 and HIF1A,as well as the protein expression levels of PTGS2 and BCL2 in gastric tissues were significantly decreased(P＜0.05),while the mRNA expression level of IL10 was significantly increased(P＜0.05).CONCLUSIONS Jiawei qifang weitong granule may ameliorate PLGC by targeted down-regulating the mRNA expression of MMP9,PTGS2,ICAM1,BCL2 and HIF1A,up-regulating IL10 mRNA expression,and regulating the imbalance between gastric mucosal inflammatory response and injury repair.关键词
加味七方胃痛颗粒/胃癌前病变/胃黏膜损伤/网络药理学/转录组学Key words
Jiawei qifang weitong granule/precancerous lesions of gastric cancer/gastric mucosal injury/network pharmacology/transcriptomics分类
医药卫生引用本文复制引用
陈文隆,唐梅文,于业平,陈鑫源,庞龙,熊常州,梁英业,王婷..加味七方胃痛颗粒改善胃癌前病变的作用机制预测与验证[J].中国药房,2026,37(15):1948-1953,6.基金项目
国家自然科学基金地区科学基金项目(No.82360959) （No.82360959）
广西自然科学基金重点项目(No.2024GXNSFDA010021) （No.2024GXNSFDA010021）
广西自然科学基金青年科学基金项目(No.2024GXNSFBA010160) （No.2024GXNSFBA010160）
