中国药房2026，Vol.37Issue(15)：1992-1997,6.DOI:10.6039/j.issn.1001-0408.2026.15.10
参苓白术散调控菌群代谢相关蛋白和TLR4/NF-κB/NLRP3炎症轴改善溃疡性结肠炎的机制研究
Mechanism of Shenling baizhu san in ameliorating ulcerative colitis via regulating flora metabolism-related proteins and TLR4/NF-κB/NLRP3 inflammatory axis
摘要
Abstract
OBJECTIVE To investigate the mechanism of Shenling baizhu san(SLBZS)in ameliorating ulcerative colitis(UC).METHODS Mice were randomly divided into normal group,model group,and low-,medium-,high-dose SLBZS groups(3,6,12 g/kg),with 10 mice in each group.Except for the normal group,mice in other groups were given free access to 3%dextran sulfate sodium solution for 7 days to establish UC models.Simultaneously,mice in each group were intragastrically administered corresponding medicinal liquid or double-distilled water once a day for consecutive 7 days.After the last administration,the histopathological morphology of colon tissues was observed;the serum levels of inflammatory factors including tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and IL-18 were detected;the total bile acid contents in liver,gallbladder and colon tissues were determined;the expression level of NOD-like receptor family pyrin domain-containing protein 3(NLRP3)in colon tissues was tested.The expression levels of flora metabolism-related proteins[G protein-coupled receptor 41(GPR41),GPR43,farnesoid X receptor(FXR),G protein-coupled bile acid receptor 1(TGR5),flavin-containing monooxygenase 3(FMO3),cytochrome P450 family 27 subfamily A member 1(CYP27A1),CYP7A1]in liver or colon tissues,as well as the proteins related to Toll-like receptor 4(TLR4)/nuclear factor-κB(NF-κB)/NLRP3 inflammatory axis[TLR4,NF-κB,phosphorylated NF-κB(p-NF-κB),myeloid differentiation factor 88(MyD88),NLRP3,apoptosis-associated speck-like protein containing a CARD(ASC),caspase-1]in colon tissues were measured.RESULTS Compared with the model group,the colon tissue injury and inflammatory cell infiltration were alleviated in medium-and high-dose SLBZS groups.The serum levels of TNF-α,IL-1β and IL-18,total bile acid contents in liver and colon tissues,as well as the protein expression levels of FMO3 and CYP7A1 in liver tissues,TLR4,p-NF-κB,NF-κB,MyD88,NLRP3,ASC and caspase-1 in colon tissues were significantly decreased(P＜0.05).Meanwhile,the total bile acid content in gallbladder,the protein expression levels of GPR41 and GPR43 in colon tissues,along with CYP27A1,FXR and TGR5 in liver tissues were significantly increased(P＜0.05).CONCLUSIONS SLBZS may relieve colonic inflammatory injury and ameliorate UC by regulating the expression of flora metabolism-related proteins and inhibiting the abnormal activation of TLR4/NF-κB/NLRP3 inflammatory axis.关键词
参苓白术散/溃疡性结肠炎/TLR4/NF-κB/NLRP3炎症轴/肠道菌群/胆汁酸代谢Key words
Shenling baizhu san/ulcerative colitis/TLR4/NF-κB/NLRP3 inflammatory axis/intestinal flora/bile acid metabolism分类
医药卫生引用本文复制引用
徐佳佳,韩飞,游宇,王佼曼,刘玉晖,常诗瑶,贺智贤,李萍,王紫嫣,熊魏..参苓白术散调控菌群代谢相关蛋白和TLR4/NF-κB/NLRP3炎症轴改善溃疡性结肠炎的机制研究[J].中国药房,2026,37(15):1992-1997,6.基金项目
江西省自然科学基金面上项目(No.20232BAB206169) （No.20232BAB206169）
江西省自然科学基金重点项目(No.20232ACB206058,No.20232ACB206064) （No.20232ACB206058,No.20232ACB206064）
江西省中医药管理局科技计划项目(No.2024B0037) （No.2024B0037）
江西省研究生创新专项资金项目(No.YC2025-S208) （No.YC2025-S208）
