中国中西医结合外科杂志2026，Vol.32Issue(4)：478-482,5.DOI:10.3969/j.issn.1007-6948.2026.04.003
铜离子电化学疗法通过诱导产生Caspase-3介导的细胞凋亡促进混合痔的痔区血管萎缩与闭塞的机制
Electro-chemical therapy by cupric ion promotes vascular atrophy and occlusion in mixed hemorrhoids through caspase-3-mediated apoptosis
摘要
Abstract
Objective To investigate the effects of electro-chemical therapy by cupric ion(ECTCI),based on the theory of necrotizing hemorrhoid ligation in Traditional Chinese Medicine,on the vascular structure and apoptotic pathways in the hemorrhoidal tissues of patients with mixed hemorrhoids,and to elucidate its therapeutic mechanism.Methods Forty patients with mixed hemorrhoids were selected and randomly assigned to a control group and three ECTCI treatment groups with different treatment durations(280 s,560 s,840 s).Hematoxylin and eosin(HE)staining was used to observe morphological changes in blood vessels.Immunohistochemistry was performed to detect the expression of CD31 and VEGF.RT-PCR and Western blot were employed to analyze the mRNA and protein expression levels of the apoptosis-related proteins Caspase-3 and Bcl-2.Results The ECTCI treatment groups exhibited dose-dependent destruction of vascular structures,manifested as reduced microvessel density,ruptured vessel walls,and fibrinoid necrosis.No significant difference in VEGF expression was observed among the groups.Apoptosis-related analyses revealed that Caspase-3 expression was up-regulated with increasing treatment duration,while Bel-2 expression was down-regulated,suggesting activation of the mitochondrial apoptotic pathway.Conclusion ECTCI promotes the atrophy and occlusion of pathological vessels by directly damaging the vascular endothelial structure in hemorrhoidal tissues and activating the Caspase-3-mediated apoptotic pathway,which represents a key mechanism in its treatment of mixed hemorrhoids.关键词
痔/铜离子电化学疗法/细胞凋亡/血管重塑/半胱天冬氨酸蛋白酶-3Key words
Hemorrhoids/electro-chemical therapy by cupric ion/apoptosis/vascular remodeling/Caspase-3分类
医药卫生引用本文复制引用
汪敏,谢振年,安晓静,蔡梦涵,赵卫兵,贾泽媛,甘沛艳..铜离子电化学疗法通过诱导产生Caspase-3介导的细胞凋亡促进混合痔的痔区血管萎缩与闭塞的机制[J].中国中西医结合外科杂志,2026,32(4):478-482,5.基金项目
国家自然科学基金(81072813) （81072813）
中国中医科学院科技创新工程(C12021A02116) （C12021A02116）
北京市海淀区卫生健康发展科研培育计划(2025XLA065-2) （2025XLA065-2）
