中国中医药科技2026，Vol.33Issue(3)：233-240,8.
基于TGF-β1/Smad3 信号通路探讨三七皂苷对脑血栓的治疗作用
Exploring the Therapeutic Effect of Panax Notoginseng Saponins on Cerebral Thrombosis Based on the TGF-β1/Smad3 Signaling Pathway
摘要
Abstract
Objective:To investigate the therapeutic effect of notoginsenoside on cerebral thrombosis through TGF-β1/Smad3 pathway.Methods:A rat model of middle cerebral artery occlusion(MCAO)was established.A total of 60 SD rats were randomly divided into six groups:blank group,model group,Naoxintong Capsule group(432 mg/kg),atorvastatin group(1.8 mg/kg),and low-dose(25 mg/kg),medium-dose(50 mg/kg)and high-dose(100 mg/kg)Panax notoginseng saponins groups.Another 10 rats were assigned to the sham operation group.Rats in each group were given intragastric administration for 4 consecutive weeks,and the blank group was given normal saline.Open field test and Morris water maze test were performed to evaluate neurological injury by detecting indicators including linearity,average speed and incubation period.Laser Doppler flowmetry was used to determine local cerebral blood flow.The serum levels of tissue-type plasminogen activator(t-PA)and plasminogen activator inhibitor-1(PAI-1)were measured by ELISA.Surface plasmon resonance(SPR)assay was adopted to detect the binding affinity between transforming growth factor-β1(TGF-β1)and Panax notoginseng saponins.TTC staining was used to calculate the percentage of cerebral infarction volume.Immunohistochemistry(IHC)and RT-qPCR were applied to detect the differential expression of TGF-β1 in brain tissue at protein localization and gene transcription levels,respectively.Results:Behavioral results showed that compared with the model group,behavioral indexes such as average speed,activity frequency,rest time and linearity were significantly improved in Panax notoginseng saponins groups(P<0.05).The serum level of t-PA was extremely significantly decreased,while PAI-1 level was increased in Panax notoginseng saponins groups(P<0.01).The mRNA expression of TGF-β1 in brain tissue was extremely significantly down-regulated in the high-dose Panax notoginseng saponins group(P<0.01).The protein expression of TGF-β1 in brain tissue was extremely significantly reduced in all dose groups of Panax notoginseng saponins(P<0.01).Conclusion:Panax notoginseng saponins can improve neurological function in rats with cerebral infarction,and treat cerebral thrombosis by regulating the levels of t-PA/PAI-1 and inhibiting the expression of TGF-β1.关键词
脑血栓/三七皂苷/转化生长因子-β1/组织型纤溶酶原激活剂/纤溶酶原激活剂抑制剂-1Key words
Panax Notoginseng Saponins/cerebral thrombosis/TGF-β1/t-Pa/PAI-1引用本文复制引用
葛学林,蒙艳丽,许庆瑞,王冬,薛岩,董羽佳,常艳宾,尹俪桥,张海峰,梁爽,王伟明..基于TGF-β1/Smad3 信号通路探讨三七皂苷对脑血栓的治疗作用[J].中国中医药科技,2026,33(3):233-240,8.基金项目
国家中药材产业技术体系专项项目(CARS-21) （CARS-21）
2023 年全国中药特色技术传承人才培训项目(T20234832005) （T20234832005）
黑龙江省自然科学基金项目(LH2023H076) （LH2023H076）
