中国中医药信息杂志2026，Vol.33Issue(8)：6-13,8.DOI:10.19879/j.cnki.1005-5304.202603548
基于网络药理学及实验验证探讨桂枝茯苓丸防治动脉型肺动脉高压的作用机制
Exploration on the Mechanism of Guizhi Fuling Pills in Preventing and Treating Pulmonary Arterial Hypertension Based on Network Pharmacology and Experimental Validation
摘要
Abstract
Objective To explore the mechanism of Guizhi Fuling Pills in preventing and treating pulmonary arterial hypertension(PAH)by integrating network pharmacology prediction,molecular docking,and animal experimental validation.Methods The active components of Guizhi Fuling Pills and their targets were screened through the TCMSP database.PAH targets were obtained from GeneCards and OMIM databases.The intersection of drug targets and disease targets was performed to construct a protein-protein interaction network and screen core targets.GO and KEGG enrichment analyses were conducted,and molecular docking was performed between key components of the drug and core targets.A monocrotaline-induced pulmonary hypertension rat model was established.Totally 40 SD rats were divided into control group,model group,sildenafil group,and Guizhi Fuling Pills low-and high-dosage groups,with 8 rats in each group.After 3 weeks of intervention,hemodynamic and morphological indices were evaluated,and qRT-PCR was used to detect the relative expressions of IL-1β,IL-6,IL-17A,and TNF-α mRNA in lung tissues.Results A total of 213 drug targets of Guizhi Fuling Pills and 9 706 disease targets of PAH were retrieved and screened,with 192 intersection targets identified.The core pathway of Guizhi Fuling Pills in treating PAH was the IL-17 signaling pathway.Molecular docking showed that the binding energies of the key active components with core targets IL1B,IL6 and TNF were all less than-5.0 kcal/mol.Animal experimental results showed that compared with the control group,rats in the model group exhibited elevated right ventricular systolic pressure and right ventricular hypertrophy,with obvious pulmonary vascular remodeling,the expressions of IL-1β,IL-6,IL-17A and TNF-α mRNA in lung tissues of rats in the model group significantly increased(P<0.000 1).Compared with the model group,right ventricular systolic pressure was significantly reduced in the Guizhi Fuling Pills high-dosage group,right ventricular hypertrophy was alleviated,pulmonary vascular remodeling was improved,the expressions of IL-1β,IL-6,IL-17A and TNF-α mRNA in lung tissues decreased(P<0.01,P<0.001,P<0.000 1).Conclusion Guizhi Fuling Pills may improve pulmonary vascular remodeling and exert preventive and therapeutic effects on PAH by regulating the IL-17 signaling pathway.关键词
动脉型肺动脉高压/桂枝茯苓丸/网络药理学/IL-17信号通路Key words
pulmonary arterial hypertension/Guizhi Fuling Pills/network pharmacology/IL-17 signaling pathway分类
医药卫生引用本文复制引用
陆天逊,郑培永,鹿振辉,顾元烨,崔洁,马双双..基于网络药理学及实验验证探讨桂枝茯苓丸防治动脉型肺动脉高压的作用机制[J].中国中医药信息杂志,2026,33(8):6-13,8.基金项目
国家自然科学基金面上项目(82374349) （82374349）
国家自然科学基金青年科学基金项目(82505469) （82505469）
上海中医药大学科技发展项目(24KFL053) （24KFL053）
