中国中医药信息杂志2026，Vol.33Issue(8)：64-70,7.DOI:10.19879/j.cnki.1005-5304.202601453
基于HOXA-AS3/miR-29a-3p/LTβR/NF-κB轴探讨健脾通络解毒方改善慢性萎缩性胃炎作用机制
Exploration on the Mechanism of Jianpi Tongluo Jiedu Prescription on Chronic Atrophic Gastritis Based on HOXA-AS3/miR-29a-3p/LTβR/NF-κB Axis
摘要
Abstract
Objective To investigate the mechanism of Jianpi Tongluo Jiedu Prescription in intervening in chronic atrophic gastritis(CAG)based on HOXA-AS3/miR-29a-3p/LTβR/NF-κB axis.Methods A CAG model was induced in rats using a compound modeling method involving 1-methyl-3-nitro-1-nitrosoguanidine combined with ranitidine.The rats were randomly divided into the following groups:blank group,model group,Weifuchun Capsule group and Jianpi Tongluo Jiedu Prescription low-,medium-,high-dosage groups.The intervention lasted for 10 weeks.Pathological changes in gastric tissue were observed via HE staining,the expression of genes and proteins related to the HOXA-AS3/miR-29a-3p/LTβR/NF-κB axis in gastric tissue were measured using RT-PCR and Western blot,respectively,serum contents of IL-1β,IL-18,IL-10,GAS and MTL were detected via ELISA.Results Compared with the blank group,the model group exhibited gastric mucosal gland atrophy and extensive inflammatory cell infiltration,the contents of IL-1β,IL-18,GAS in serum significantly increased(P<0.01),the content of IL-10 and MTL significantly decreased(P<0.01),the mRNA expressions of HOXA-AS3,LTβR,NF-κB p65,NLRP3,IL-1β and IL-18 mRNA in gastric tissue significantly increased(P<0.05),the expression of miR-29a-3p significantly decreased(P<0.01),and the protein expressions of LTβR,p-IκBα,p-NF-κB p65,Caspase-1 and NLRP3 in gastric tissue significantly increased(P<0.01);after intervention with different dosages of Jianpi Tongluo Jiedu Prescription,pathological damage to the gastric mucosa was alleviated to varying degrees,the contents of IL-1β,IL-18,GAS in serum decreased,the content of IL-10 and MTL increased,the expression of mRNA and proteins related to the HOXA-AS3/miR-29a-3p/LTβR/NF-κB axis were reversed to varying degrees,among which Jianpi Tongluo Jiedu Prescription high-dose group showed statistical significance(P<0.05,P<0.01).Conclusion Jianpi Tongluo Jiedu Prescription can effectively improve pathological damage in gastric mucosal tissues of CAG rats,and its mechanism may be associated with the regulation of the HOXA-AS3/miR-29a-3p/LTβR/NF-κB axis.关键词
慢性萎缩性胃炎/健脾通络解毒方/大鼠/HOXA-AS3/miR-29a-3p/LTβR/NF-κB轴Key words
chronic atrophic gastritis/Jianpi Tongluo Jiedu Prescription/rats/HOXA-AS3/miR-29a-3p/LTβR/NF-κB axis分类
医药卫生引用本文复制引用
李萌,罗楠,易柳凤,丁媛,于靖文,姜天童,王少丽,刘震..基于HOXA-AS3/miR-29a-3p/LTβR/NF-κB轴探讨健脾通络解毒方改善慢性萎缩性胃炎作用机制[J].中国中医药信息杂志,2026,33(8):64-70,7.基金项目
国家自然科学基金面上项目(82174352) （82174352）
中央级公益性科研院所基本科研业务费专项资金(ZZ19-XRZ-047) （ZZ19-XRZ-047）
