智慧农业导刊2026，Vol.6Issue(14)：30-34,5.DOI:10.20028/j.zhnydk.2026.14.008
基于气相色谱-质谱法测定大米中2-乙酰基-1-吡咯啉含量的实验项目探索与实践
摘要
Abstract
Expanding the experimental teaching project for determining 2-Acetyl-1-pyrroline in Rice by GC-MS in the agri-cultural product quality analysis course.Through the optimization of sample pretreatment methods,mass spectrometry conditions,and methodological comparison and validation,a reliable gas chromatography-mass spectrometry(GC-MS)method for quantifying 2-acetyl-1-pyrroline in rice was successfully established.The results showed that:2-acetyl-1-pyrroline in rice exhibited an ex-cellent linear correlation(R2=0.9999)within the range of 15~1 000 ng/mL.The precision,expressed as relative standard deviation(RSD),ranged from 0.64%to 1.09%.The spike recovery rates were between 87.2%and 98.8%.The method's limit of detection(LOD)and limit of quantitation(LOQ)were determined to be 0.005 μg/g and 0.015 μg/g,respectively,which meets the acceptable criteria for trace analysis.The developed method proved to be operable,sensitive,and precise,making it suitable for the rapid and accurate determination of 2-acetyl-1-pyrroline in rice.Furthermore,this experimental project,with its appropriate level of difficulty,is effective in stimulating students'scientific interest and fostering their innovative thinking and spirit of research explo-ration.关键词
气相色谱-质谱法/大米/2-乙酰基-1-吡咯啉/实验项目/探索Key words
gas chromatography-mass spectrometry(GC-MS)/rice/2-acetyl-1-pyrroline/experimental project/exploration分类
医药卫生引用本文复制引用
路丹,梁琼月,韦燕燕,刘燕梅,沈方科,韦婷彦,杨娟,农梦玲,范晓苏..基于气相色谱-质谱法测定大米中2-乙酰基-1-吡咯啉含量的实验项目探索与实践[J].智慧农业导刊,2026,6(14):30-34,5.基金项目
广西壮族自治区级"四新"研究与实践项目(XNK202401) （XNK202401）
广西高等教育本科教学改革工程项目(2025JGA111) （2025JGA111）
广西学位与研究生教育改革课题(JGY2023033,JCY2025027) （JGY2023033,JCY2025027）
广西大学本科教学改革工程项目(JG2024YB057,JG2025YB041) （JG2024YB057,JG2025YB041）
广西大学实验教学改革项目(syjg2025034,syjg2025033) （syjg2025034,syjg2025033）
