种子2026，Vol.45Issue(7)：28-40,13.DOI:10.16590/j.cnki.1001-4705.2026.07.028
基于转录组测序的人参成花基因挖掘及激素关联分析
Gene Mining and Hormone Correlation Analysis of Panax ginseng Flowering based on Transcriptome Sequencing
摘要
Abstract
To investigate the differentially expressed genes(DEGs)and endogenous hormone changes associated with flowering in Panax ginseng and to identify key regulatory factors,leaves from flow-ering and non-flowering plants of 3 Panax ginseng lines were used as materials.High-throughput transcriptome sequencing was employed for comparative transcriptomic analysis,endogenous hor-mone contents were determined using liquid chromatography-tandem mass spectrometry(LC-MS/MS),and the expression of key genes was validated by qRT-PCR.A total of 63 819 genes were ob-tained,and 2 365 DEGs related to flowering were identified.GO and KEGG enrichment analyses showed that the DEGs were significantly enriched in pathways such as secondary metabolite biosyn-thesis and plant hormone signal transduction.WGCNA identified a blue core module that was signifi-cantly positively correlated with gibberellin and cytokinin.PPI network analysis identified LFY,HTR2,and F24B9.25 as core hub genes in the flowering regulatory network.The qRT-PCR valida-tion results were consistent with the transcriptome sequencing data.This study confirmed that genes such as LFY,HTR2,and F24B9.25 were upregulated in flowering plants of Panax ginseng,revea-ling the potential key roles of gibberellin and cytokinin signaling pathways in the regulation of flower-ing in Panax ginseng.关键词
人参/成花/转录组测序/差异表达基因/内源激素Key words
Panax ginseng/flowering/transcriptome sequencing/differentially expressed genes/endogenous hormones分类
农业科技引用本文复制引用
胡慧,荣俊博,冯历文,吴佳,江怡洁,王友程,付春悦,雷秀娟,王英平..基于转录组测序的人参成花基因挖掘及激素关联分析[J].种子,2026,45(7):28-40,13.基金项目
国家重点研发项目"人参等道地药材品种资源收集、筛选及繁育技术研究与示范"(2021YFD1600901) （2021YFD1600901）
国家自然科学基金联合基金项目"人参重要产量和品质性状遗传解析及分子设计育种基础研究"(U21A20405) （U21A20405）
"长白英才计划"杰出人才项目"杰出人才团队"(202410007) （202410007）
