种子2026，Vol.45Issue(7)：72-79,8.DOI:10.16590/j.cnki.1001-4705.2026.07.072
不同类型钠盐胁迫对玉米自交系B73萌发的影响
The Effects of Different Types of Sodium Salt Stress on Germination of Maize Inbred Line B73
摘要
Abstract
The germination stage is the most sensitive phase of maize(Zea mays L.)in response to salt stress,and elucidating its salt tolerance mechanisms is of great significance for stress-resistant breed-ing in maize.Using the maize inbred line B73 as the material,the germination indicators,physiologi-cal characteristics,and expression changes of salt stress-responsive genes were analyzed under treat-ments with different concentrations of neutral salts(NaCl,Na2SO4)and alkaline salts(NaHCO3,Na2CO3).The results showed that the germination indicators of maize changed significantly with in-creasing salt concentrations.Seed germination was markedly inhibited under 150 mmol/L stress for all sodium salts,with Na2CO3 exhibiting the most prominent inhibitory effect.The germination vigor,germination percentage,and germination index decreased to 86.3％,81.8％,and 84.7％of the con-trol,respectively,and the salt injury index increased to 85％.The activities of antioxidant enzymes in the radicle and plumule displayed distinct tissue specificity and salt dependency;the activities of SOD,POD,CAT,and APX in the radicle were significantly inhibited,with alkaline salt stress being the most pronounced.Overall,alkaline salt stress caused significantly greater damage to plants than neu-tral salt stress,and different types of sodium salts at 150 mmol/L showed significant differences com-pared with the control.Expression analysis of salt stress-related genes revealed that ZmSOD3 was highly significantly upregulated under NaCl and NaHCO3 stress,ZmPOD,ZmPOD44,ZmPRX1,and ZmCAT1 were highly significantly upregulated under NaHCO3 stress,ZmGT61 WTBZ and ZmMAPK4 were significantly upregulated under Na2CO3 stress,and ZmMAC39 and ZmMAC82 were significantly downregulated under salt treatments.In conclusion,the inhibition of maize seed germination by sodium salt stress was concentration-dependent,with Na2CO3 stress exhibiting the most significant effect,and gene expression regulation was specific to different sodium salt stresses.关键词
玉米/萌发期/自交系/盐胁迫Key words
Zea mays L./germination stage/inbred lines/saline stress分类
农业科技引用本文复制引用
胡胜男,杨溪林,刘丽滨,王霞,孙丽芳,何长安..不同类型钠盐胁迫对玉米自交系B73萌发的影响[J].种子,2026,45(7):72-79,8.基金项目
黑龙江省玉米产业技术协同创新推广体系 （）
黑龙江省农业科学院创新工程资助项目(CX23ZD05) （CX23ZD05）
