种子2026，Vol.45Issue(7)：84-91,145,9.DOI:10.16590/j.cnki.1001-4705.2026.07.084
向日葵不同器官响应列当寄生的转录组分析
Transcriptome Analysis of Different Organs in Helianthus annuus in Response to Orobanche Parasitism
摘要
Abstract
The damage caused by Orobanche parasitism on Helianthus annuus has been increasing year by year,severely restricting the improvement of sunflower yield and quality.To elucidate the molecu-lar regulatory mechanisms and metabolic pathways underlying the response of Helianthus annuus to Orobanche parasitism,the locally dominant cultivar SH361 was used as the experimental material.An Orobanche-infected group and a normal growth control group were established,and transcriptome se-quencing(RNA-seq)combined with bioinformatics analysis was employed to investigate the gene ex-pression patterns in 5 organs:roots,stems,leaves,petals,and seeds.The results showed that a total of 219.24 Gb of clean data was obtained,with GC contents ranging from 44.34％to 45.47％,and Q20 and Q30 base percentages not lower than 98.06％and 94.10％,respectively,indicating high sequen-cing data quality.Comparative analysis identified 1 531,2 314,2 290,2 137,and 1 658 differentially expressed genes(DEGs)in the respective organs,with the fewest DEGs in roots and the most in stems.KEGG pathway enrichment analysis of the DEGs revealed that the MAPK signaling pathway-plant was the core enriched pathway,and the pathway enrichment characteristics differed markedly a-mong organs.These DEGs synergistically mediate the defense response of Helianthus annuus to Oro-banche parasitism by regulating mRNA transcription and the biosynthesis and metabolism of resist-ance-related hormones.关键词
向日葵/列当/转录组分析/差异表达基因/功能富集Key words
Helianthus annuus/Orobanche parasitism/transcriptome analysis/differentially expressed genes(DEGs)/functional enrichment分类
农业科技引用本文复制引用
王海燕,崔超,燕晓慧,张惠..向日葵不同器官响应列当寄生的转录组分析[J].种子,2026,45(7):84-91,145,9.基金项目
内蒙古自治区自然科学基金项目(2021MS03030) （2021MS03030）
内蒙古自治区高等学校科学研究重点项目(NJZZ21060) （NJZZ21060）
内蒙古自治区高等学校科学研究项目(NJZY19244) （NJZY19244）
