浙江大学学报（医学版）2026，Vol.55Issue(5)：404-413,10.DOI:10.3724/zdxbyxb-2025-0418
菟丝子能减少阿尔茨海默病模型细胞的氧化损伤
Cuscuta chinensis Lam.reduces oxidative injury in Alzheimer's disease model cells
摘要
Abstract
Objective:To investigate the protective effect of Cuscuta chinensis Lam.against oxidative injury in an Alzheimer's disease(AD)cell model,as well as the related molecular mechanism.Methods:Ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry was used to analyze and identify the chemical components of Cuscuta chinensis Lam..An oxidative injury model was established in HT22 neuronal cells treated with Aβ1-42.Cell viability was measured by CCK-8 assay,and apoptosis was detected by Annexin V-FITC/PI double staining.Intracellular reactive oxygen species(ROS)levels were detected using the DCFH-DA probe,and the expression of antioxidant-and apoptosis-related proteins was examined by Western blotting.Results:A total of 381 chemical components were identified from Cuscuta chinensis Lam.,among which astragalin,hyperoside,kaempferol,and quercetin were the main constituents.Cell experi-ments showed that Cuscuta chinensis Lam.at concentrations of 0.50,0.75,and 1.00 mg/mL increased the viability of Aβ1-42-treated HT22 cells,decreased the apoptosis rate,inhibited ROS excessive accumulation,upregulated the expression of the antioxidant-related proteins heme oxygenase-1 and NQO1,downregulated the expression of the apoptosis-related protein cytochrome C,and reduced the ratios of activated caspase-3/caspase-3 and BAX/Bcl-2.Conclusion:Cuscuta chinensis Lam.may attenuate oxidative injury in AD model cells by activating the endogenous antioxidant defense system and inhibiting the mitochondrial-dependent apoptotic pathway.关键词
阿尔茨海默病/菟丝子/氧化应激/线粒体凋亡/HT22细胞Key words
Alzheimer's disease/Cuscuta chinensis Lam./Oxidative stress/Mitop-tosis/HT22 cell分类
医药卫生引用本文复制引用
李德雨,刘欣,陆韵薇,于顾然..菟丝子能减少阿尔茨海默病模型细胞的氧化损伤[J].浙江大学学报（医学版）,2026,55(5):404-413,10.基金项目
国家重点研发计划(2022YFC3501403) （2022YFC3501403）
国家自然科学基金(82405265) （82405265）
江苏省院士工作站(BM2024101) （BM2024101）
广东省基础与应用基础研究基金(2023A1515110832) （2023A1515110832）
深圳市科技计划(JCYJ20230807094801002)This study was supported by National Key R&D Program of China(2022YFC3501403),National Natural Science Foundation of China(82405265),Jiangsu Provincial Academician Workstation(BM2024101),Guangdong Basic and Applied Basic Research Foundation(2023A1515110832),and Shenzhen Science and Technology Program(JCYJ20230807094801002) （JCYJ20230807094801002）
