作物学报2026，Vol.52Issue(8)：2244-2256,13.DOI:10.3724/SP.J.1006.2026.61025
小麦铜转运蛋白基因TaCOPT3响应镉胁迫的功能分析
Functional analysis of the wheat copper transporter gene TaCOPT3 in response to cadmium stress
摘要
Abstract
Copper transporters(COPT)are involved in maintaining metal ion homeostasis in plants,but their role in the response of wheat to cadmium(Cd)stress remains unclear.Based on previous transcriptome analysis revealing that TaCOPT3 is signifi-cantly upregulated under Cd stress,this study cloned its three homologous copies and systematically analyzed their sequence characteristics,expression patterns,and biological functions.Sequence analysis showed that TaCOPT3-A,TaCOPT3-B,and Ta-COPT3-D encode 162,150,and 162 amino acids,respectively.All contain the conserved Ctr copper transport domain and share 65.40%-68.67%sequence identity with OsCOPT3.Subcellular localization prediction showed that all three copies of TaCOPT3 were localized to the plasma membrane.The promoter regions were rich in hormone-responsive elements(ABA,MeJA,and SA)and MYB transcription factor binding sites.RT-qPCR analysis showed that Cd stress significantly increased the expression of all three TaCOPT3 copies,with levels rising as Cd concentration increased.Under 300 μmol L-1 CdCl2 treatment,the expression level of TaCOPT3-A in the shoot was 427 times higher than that of the control,which was higher than that of other copies.Func-tional analysis showed that under 50 μmol L-1 and 300 μmol L-1 CdCl2 treatment,shoot Cd accumulation in the TaCOPT3-A mu-tant e0483 was significantly reduced by 44.52%and 30.92%,respectively,compared with the wild type,and the bioconcentration factor(BCF)was significantly reduced by 36.62%and 24.19%,respectively.Haplotype analysis showed that TaCOPT3-A could be divided into two main haplotypes,with haplotype 1 accounting for 63.30%of global wheat germplasm.The known low-Cd-accumulating variety Jimai 22 belongs to this haplotype.Taken together,TaCOPT3 is a Cd-responsive gene in wheat.Loss of function of TaCOPT3-A significantly reduces Cd accumulation in shoots,suggesting its role in regulating Cd transport to the aboveground tissues.These findings provide a theoretical basis for unraveling the mechanism of wheat response to Cd stress.关键词
小麦/Cd胁迫/TaCOPT3/铜转运蛋白/突变体/单倍型Key words
wheat/Cd stress/TaCOPT3/copper transporter/mutant/haplotype引用本文复制引用
贾康楠,李慧园,郭会君,赵林姝,谢永盾,熊宏春,古佳玉,张玉梅,刘录祥..小麦铜转运蛋白基因TaCOPT3响应镉胁迫的功能分析[J].作物学报,2026,52(8):2244-2256,13.基金项目
本研究由核技术研发科研项目(HJSYF2024(31)),山东省重点研发计划项目(2024LZGCQY001)和北京市"高创计划"青年人才托举工程(20250783)资助.This study was supported by the Nuclear Technology Research and Development Project(HJSYF2024(31)),the Shandong Provincial Key Research and Development Program Project(2024LZGCQY001),and the Young Elite Scientists Sponsorship Program of the Beijing High Innovation Plan(20250783). （HJSYF2024(31）
