作物学报2026，Vol.52Issue(8)：2268-2278,11.DOI:10.3724/SP.J.1006.2026.65002
芥菜型油菜耐旱相关miRNA的挖掘及功能研究
Identification of miRNAs response to drought stress and function research in Brassica juncea
摘要
Abstract
Due to the influence of karst landforms,Guizhou is more susceptible to drought under extreme weather events,posing a serious threat to rapeseed production.Compared to Brassica napus,Brassica juncea exhibits greater drought tolerance in pro-duction.Therefore,mining drought-tolerant-related genes and elucidating their molecular functions is of great significance for improving stress resistance and yield in rapeseed.MicroRNAs(miRNAs)have been shown to play critical regulatory roles in plant drought stress response,yet studies in B.juncea remain limited.In this study,two B.juncea lines with contrasting drought responses(G160 and G170)were used.Combined small RNA(sRNA)sequencing and molecular functional assays were per-formed to predict and functionally analyze drought-responsive miRNAs and their target genes.A total of 44 known and novel drought-responsive miRNAs were identified.RT-qPCR validated the dynamic expression patterns of eight miRNAs under drought stress,revealing that their expression levels were lower in the drought-tolerant line G170 than in the sensitive line G160.Target gene prediction indicated that some target genes are involved in drought response(e.g.,ATHBs,PCL5)and ABA signaling path-ways(e.g.,AHA1,CAR9).Furthermore,heterologous overexpression of BjuMIR9552a in B.napus was performed.Drought-rehydration tests and physiological/biochemical measurements showed that transgenic plants exhibited lower drought tolerance than wild-type plants.RT-qPCR analysis revealed significant downregulation of AHA family genes in the transgenic lines,suggesting that BjuMIR9552a may negatively regulate drought resistance in rapeseed by targeting AHA family genes.This study identified eight miRNAs that may act as repressors of drought stress;overexpression of BjuMIR9552a reduces drought resistance and downregulates AHA family gene expression.These findings provide a new theoretical basis for further dissecting the miRNA-mediated drought response regulatory network in B.juncea.关键词
芥菜型油菜/miRNA/干旱胁迫/靶基因/转基因Key words
Brassica juncea/miRNA/drought stress/target gene/transgenic引用本文复制引用
蒋唤唤,王璐璐,张超,张玉玲,梁峰豪,唐容,杨斌,肖华贵,雷绍林,陶源,戴丽红..芥菜型油菜耐旱相关miRNA的挖掘及功能研究[J].作物学报,2026,52(8):2268-2278,11.基金项目
本研究由贵州省农业科学院青年博士基金项目(黔农科博士基金[2024]05 号),贵州省自然科学基金项目(黔科合基础-ZK[2024]一般566 和黔科合基础-ZK[2024]一般 560),贵州省农业科学院"十五五"学科团队建设项目(黔农科院新兴团队[2026]08)和国家自然科学基金项目(32360492)资助.This study was supported by the Doctoral Fund of Guizhou Academy of Agricultural Sciences(Qiannongke Doctoral Fund[2024]05),the Guizhou Provincial Natural Science Research Program(Qiankehejichu-ZK[2024]general 566 and Qiankehejichu-ZK[2024]general 560),the Discipline Team Construction of Guizhou Academy of Agricultural Sciences for"15th Five-Year Plan"(Qiannongkeyuan Emerging Team[2026]08),and the National Natural Science Foundation of China(32360492). （黔农科博士基金[2024]05 号）
