作物学报2026，Vol.52Issue(8)：2306-2316,11.DOI:10.3724/SP.J.1006.2026.54155
绿豆柱头外露突变体se2的特征特性分析及候选基因挖掘
Characterization and candidate gene mining of the stigma-exsertion mutant se2 in mungbean
摘要
Abstract
Stigma-exsertion materials can facilitate cross-pollination in mungbean under natural conditions,improve the effi-ciency of hybrid seed production,and promote the breeding of new mungbean varieties with stigma exsertion.In addition,identi-fying genes that regulate stigma development will contribute to the utilization of heterosis in mungbean.In this study,the mung-bean cultivar Jilyu 7(WT)was used as the experimental material,and a genetically stable stigma-exsertion mutant,se2,was iden-tified through ethyl methanesulfonate(EMS)mutagenesis and artificial screening.Phenotypic characterization,genetic analysis,and candidate gene mining were subsequently conducted.The results showed that,compared with WT,se2 exhibited a signifi-cantly shortened keel flap,an exserted stigma,and normal pollen viability.In field experiments,the pod number per plant,seed number per pod,pod length,and yield per plant of se2 decreased by 83.4%,60.9%,25.1%,and 85.4%,respectively,compared with WT.In pot experiments,the number of flowers per plant,pod number per plant,and proportion of shrunken pods in se2 in-creased by 285.2%,285.9%,and 67.6%,respectively,whereas the number of mature pods per plant decreased by 93.6%,indica-ting a significant reduction in seed-setting rate.Segregation ratio statistical analysis of traits in the F2 population showed that the stigma-exsertion trait was controlled by a single recessive gene.The potential of se2 for mungbean hybrid breeding was further confirmed by bee-assisted pollination.After resequencing the mutant DNA bulk and the wild-type parent,six candidate genes related to stigma exsertion were identified through MutMap analysis,homologous sequence alignment,and expression analysis.This study lays a foundation for elucidating the molecular mechanism underlying stigma-exsertion development and for exploi-ting heterosis in mungbean.关键词
绿豆/柱头外露/突变体/特征特性/基因挖掘Key words
mungbean/stigma exsertion/mutant/characteristics/gene mining引用本文复制引用
徐亚鹏,郭中校,邓昆鹏,兰天娇,王明海,程钰鑫,王涵,包淑英,韩丹,徐宁..绿豆柱头外露突变体se2的特征特性分析及候选基因挖掘[J].作物学报,2026,52(8):2306-2316,11.基金项目
本研究由吉林省农业科技创新工程项目(CXGC2024ZY004)和财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-08-Z8)资助.This study was supported by the Agricultural Science and Technology Innovation Program of Jilin Province(CXGC2024ZY004)and the China Agriculture Research System of MOF and MARA(CARS-08-Z8). （CXGC2024ZY004）
