作物学报2026，Vol.52Issue(8)：2327-2335,9.DOI:10.3724/SP.J.1006.2026.55078
花生含油量分子标记开发及其在高油育种中的应用
Development of molecular markers for peanut oil content and their application in breeding for high-oil varieties
摘要
Abstract
Peanut(Arachis hypogaea L.)is an important oil crop in China,and breeding varieties with high oil content is a critical approach to enhancing industrial efficiency.Kernel oil content as a typical quantitative trait,is controlled by multiple minor-effect genes.In this study,molecular markers were developed for seven stable oil content quantitative trait loci(QTLs)repeatedly de-tected across multiple peanut populations.These markers were used to genotype 310 peanut germplasm accessions and the distri-bution patterns and pyramiding effects of high-oil alleles were systematically analyzed.The results showed that the high-oil allele at a single QTL could increase oil content by 0.38-3.61 percentage points,with phenotypic effects ranging from 53.79％to 55.47％.The frequency of high-oil alleles varied significantly among different QTLs,with qOCA05 having the lowest frequency(6％)and qOCA06.2 the highest(94％).Only two accessions were found to carry all seven high-oil alleles,with oil contents of 56.36％and 58.59％,indicating that pyramiding multiple genes can significantly increase seed oil content.Analysis revealed that all 60 accessions with oil content above 55％carried at least two high-oil alleles;among them,four accessions with oil content exceeding 60％had pyramided five to six high-oil alleles,suggesting the presence of other genetic factors with larger effects.The findings of this study provide practical molecular markers and important breeding strategies for high-oil peanut breeding.关键词
花生/含油量/数量性状位点/分子标记/聚合效应Key words
peanut/oil content/QTL/molecular marke/pyramiding effect引用本文复制引用
郎瑞博,姜慧芳,廖伯寿,雷永,淮东欣,黄莉,刘念,晏立英,陈玉宁,王欣,王前前,康彦平,王志慧..花生含油量分子标记开发及其在高油育种中的应用[J].作物学报,2026,52(8):2327-2335,9.基金项目
本研究由国家重点研发计划(2023YFD1202800),湖北省支持种业高质量发展资金项目(HBZY2023B003),岳麓山实验室种业专项项目(YLS-2025-ZY02038),中国农业科学院科技创新工程项目(2025-2060299-089-031)和中国农业科学院南繁专项(YBXM2552)资助.This study was supported by the National Key R&D Program of China(2023YFD1202800),the Project of the Development for High-quality Seed Industry of Hubei Province(HBZY2023B003),the Yuelushan Laboratory Breeding Project(YLS-2025-ZY02038),the Innovation Pro-gram of the Chinese Academy of Agricultural Sciences(2025-2060299-089-031),and the Nanfan Special Project of CAAS(YBXM2552). （2023YFD1202800）
