植物保护2026，Vol.52Issue(4)：22-36,15.DOI:10.16688/j.zwbh.2025373
本氏烟肌球蛋白NbMyosin Ⅺ-2调控番茄黄化曲叶病毒细胞间运动的分子机制
Molecular mechanism of Nicotiana benthamiana NbMyosin Ⅺ-2 in regulating the intercellular movement of tomato yellow leaf curl virus
摘要
Abstract
Tomato yellow leaf curl virus(TYLCV)is a globally distributed plant virus that causes severe damage to crops such as tomato and tobacco plants.To investigate its pathogenic mechanisms,the TYLCV-encoded movement protein V2 was used as bait to screen and identify interacting protein from Nicotiana benthamiana.A myosin motor protein,NbMyosin Ⅺ-2(GenBank:DQ875135.1),was identified as a V2-interacting partner.Bimolecular fluorescence complementation,co-localization,and co-immunoprecipitation assays confirmed that V2 directly interacts with NbMyosin Ⅺ-2,and that they form punctate complexes along actin filaments.Gene silencing assays revealed that knockdown of NbMyosin Ⅺ-2 inhibited TYLCV infection in N.benthamiana and disrupted the localization of the TYLCV movement complex V2-C5 to plasmodesmata,trapping it in the cytoplasm,without affecting the silencing suppressor activity of V2.Furthermore,transient overexpression of a dominant-negative mutant of NbMyosin Ⅺ-2 significantly inhibited infection by TYLCV,tobacco curly shoot virus,and tomato leaf curl Yunnan virus.This study indicates that NbMyosin Ⅺ-2 regulates the intercellular movement of geminiviruses through interaction with V2,providing a potential target for the development of antiviral strategies.关键词
番茄黄化曲叶病毒/运动蛋白V2/本氏烟肌球蛋白NbMyosin Ⅺ-2Key words
tomato yellow leaf curl virus(TYLCV)/movement protein V2/NbMyosin Ⅺ-2分类
农业科技引用本文复制引用
胡广敏,周雪平,李方方..本氏烟肌球蛋白NbMyosin Ⅺ-2调控番茄黄化曲叶病毒细胞间运动的分子机制[J].植物保护,2026,52(4):22-36,15.基金项目
国家自然科学基金(W2411024,32172385) （W2411024,32172385）
