植物保护2026，Vol.52Issue(4)：37-45,9.DOI:10.16688/j.zwbh.2025268
大麦条纹病菌Pg00232基因致病功能研究
Pathogenic function analysis of the Pg00232 gene in Pyrenophora graminea
摘要
Abstract
Barley leaf stripe,caused by Pyrenophora graminea,is mainly transmitted through infected seeds.In this study,bioinformatics analysis and RNA interference(RNAi)technology were utilized to preliminarily investigate the regulatory role of the Pg00232 gene in the pathogenicity of P.graminea.The results showed that the full-length Pg00232 gene is 624 bp,encoding 207 amino acids.The encoded protein contains a signal peptide and is an unstable hydrophilic protein.Subcellular localization analysis revealed that the protein is localized in both the nucleus and the cell membrane of host.Two RNAi silencing strains,Pg00232-RNAi-1 and Pg00232-RNAi-2,were successfully generated via PEG-mediated protoplast transformation.RT-qPCR analysis showed that the expression levels of Pg00232 in the two strains decreased by 30.73％and 48.92％,respectively,compared with the wild-type strain QWC.Moreover,the disease incidence of barley infected by the RNAi silencing strains was significantly reduced by 55.11％and 59.69％,respectively,compared with QWC,and their growth rates decreased by 20.00％and 24.44％,respectively(P＜0.05).Observation under an integrated upright and inverted microscope showed that the hyphae of the RNAi silencing strains were intertwined or exhibited irregular breakage.Trypan blue and DAB staining indicted that both the number of dead cells and the level of reactive oxygen species accumulation in barley leaves infected by the RNAi silencing strains were significantly reduced,in comparison with those leaves infected by QWC.These results indicate that Pg00232 not only regulates hyphal growth and development of P.graminea,but also plays a critical role in its pathogenesis,providing a theoretical basis for further dissecting the pathogenic mechanism of P.graminea.关键词
大麦条纹病菌/亚细胞定位/RNAi/致病性Key words
Pyrenophora graminea/subcellular localization/RNA interference/pathogenicity分类
农业科技引用本文复制引用
冯雪琪,侯璐,马如清,杨文娟,李妍,汪军成,孟亚雄,姚立蓉,王化俊,司二静..大麦条纹病菌Pg00232基因致病功能研究[J].植物保护,2026,52(4):37-45,9.基金项目
国家自然科学基金(32160496,32160647,32460517) （32160496,32160647,32460517）
国家大麦青稞产业技术体系(CARS-05-02A-02) （CARS-05-02A-02）
甘肃省教育厅产业支撑计划(2021CYZC-12) （2021CYZC-12）
甘肃省现代寒旱特色农业麦类产业技术体系(GSARS-070) （GSARS-070）
甘肃农业大学大学生创新创业训练项目(202501068,202501036) （202501068,202501036）
