植物保护2026，Vol.52Issue(4)：46-65,87,21.DOI:10.16688/j.zwbh.2025379
杨树HMP家族全基因组鉴定及溃疡病响应分析
Genome-wide identification of the HMP gene family in Populus trichocarpa and analysis of its response to canker
摘要
Abstract
Heavy metal-associated proteins(HMPs)are a class of proteins widely involved in the regulation of heavy metal homeostasis.They play crucial roles in plant growth and development,participating in physiological processes such as ion transport,homeostasis maintenance,and nutrient transport.Current research on HMP family members primarily focuses on their roles in metal buffering and distribution,while their expression patterns and stresses resistance mechanisms under pathogen stress remain largely unexplored.In this study,HMP family members were identified from the genome of Populus trichocarpa.Their biological characteristics,including physicochemical properties,gene structure and cis-acting elements in promoters,were comprehensively analyzed.Expression profiling under Botryosphaeria dothidea stress was performed using RNA-Seq analysis and RT-qPCR validation.The results demonstrated that 84 PtrHMPs were identified from the genome and phylogenetically classified into three subfamilies.Functional divergence among these genes was primarily driven by variations in gene structure,motif composition,26 segmental duplication events(Ka/Ks=0.27),and subfamily-specific cis-regulatory modules.Among them,48 PtrHMPs contained N-glycosylation sites,six possessed signal peptides,and four contained transmembrane helices.Cross-species collinearity analysis revealed that PtrHMPs had the highest homology with Glycine max(89 homologous gene pairs),indicating evolutionary conservation of the HMP family in dicotyledonous plants.Furthermore,PdPapHMP3,PdPapHMP16,PdPapHMP57,and PdPapHMP62 in P.davidiana × P.alba var.pyramidalis were significantly induced under B.dothidea stress.Collectively,this work systematically elucidates the biological features of PtrHMPs and their expression patterns under B.dothidea challenge,providing a theoretical basis for improving disease-resistant traits and breeding resistant poplar varieties.关键词
杨树/葡萄座腔菌/HMP家族/植物-病原互作/表达模式Key words
Populus/Botryosphaeria dothidea/HMP gene family/plant-pathogen interaction/expression patterns分类
农业科技引用本文复制引用
申东晨,林辉,刁健,马玲..杨树HMP家族全基因组鉴定及溃疡病响应分析[J].植物保护,2026,52(4):46-65,87,21.基金项目
国家重点研发计划(2022YFD1401001) （2022YFD1401001）
国家自然科学基金青年基金(32301591) （32301591）
