植物保护2026，Vol.52Issue(4)：66-75,99,11.DOI:10.16688/j.zwbh.2025421
侵染云南省滇红花的黄瓜花叶病毒的基因组特征及致病性分析
Genomic characterization and pathogenicity assessment of cucumber mosaic virus infecting safflower in Yunnan province
摘要
Abstract
Cucumber mosaic virus(CMV)belongs to the genus Cucumovirus in the family Bromoviridae and has a wide host range,infecting 1 071 species in 521 genera across 100 families.Carthamus tinctorius(safflower)is an important medicinal plant,and CMV is widely prevalent in safflower worldwide,causing severe damage.In this study,safflower plants exhibiting symptoms of chlorosis,dwarfing,and bud blight were collected in Yunnan province for virus detection.Through small RNA sequencing and RT-PCR amplification,three CMV-infcctcd plants were identified,and the complete genome sequences of CMV RNA1,RNA2,and RNA3 were obtained and designated as CMV-crocus.Sequence analysis showed that the 2b protein of CMV-crocus differed greatly from those of other CMV isolates.Further investigations revealed that satellite RNAs from other isolates could reduce the viral accumulation of CMV-crocus in safflower.This study is the first to obtain the full-length genome sequence of CMV from safflower in Yunnan and to investigate its pathogenicity,providing a basis for subsequent virus control and prevention in safflower.关键词
红花/黄瓜花叶病毒/卫星RNA/药用植物Key words
safflower/cucumber mosaic virus/satellite RNA/medicinal plant分类
农业科技引用本文复制引用
董汝,尹跃艳,钟静,赵丽玲,林涛,李婷婷,丁铭..侵染云南省滇红花的黄瓜花叶病毒的基因组特征及致病性分析[J].植物保护,2026,52(4):66-75,99,11.基金项目
云南省种子种业联合实验室项目(202205AR070001-02) （202205AR070001-02）
云南省重大科技专项计划(202302AE090005-6) （202302AE090005-6）
云南省乡村振兴科技专项(200304BI090032-9) （200304BI090032-9）
云南省农业科学院生物技术与种质资源研究所开放项目(2025002) （2025002）
